Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9ZH99

- IDH_COXBU

UniProt

Q9ZH99 - IDH_COXBU

Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    pH dependencei

    Optimum pH is 6.5-7.7.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei114 – 1141NADPBy similarity
    Binding sitei123 – 1231SubstrateBy similarity
    Binding sitei125 – 1251SubstrateBy similarity
    Binding sitei129 – 1291SubstrateBy similarity
    Binding sitei139 – 1391SubstrateBy similarity
    Binding sitei163 – 1631SubstrateBy similarity
    Sitei170 – 1701Critical for catalysisBy similarity
    Sitei240 – 2401Critical for catalysisBy similarity
    Metal bindingi317 – 3171Magnesium or manganeseBy similarity
    Binding sitei362 – 3621NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei401 – 4011NADPBy similarity
    Binding sitei405 – 4051NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi349 – 3557NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-1188-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:icd
    Ordered Locus Names:CBU_1200
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Isocitrate dehydrogenase [NADP]PRO_0000323713Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei123 – 1231PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9ZH99.

    Expressioni

    Developmental stagei

    Detected in both the small cell variant (SCV) and in the large cell variant (LCV) stage (at protein level). LCVs are more metabolically active than SCVs.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZH99.
    SMRiQ9ZH99. Positions 12-426.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK00031.
    OMAiCLRPIRY.
    OrthoDBiEOG6SNDTP.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004439. Isocitrate_DH_NADP_dimer_prok.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZH99-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTELTGVSIV TYQHIKVPSQ GEKITVNKAV LEVPDRPIIP FIEGDGIGID    50
    IAPVMKNVVD AAVEKSYAGK RKIEWMEIYA GEKATKVYGK DNWLPDETLE 100
    AIKEYQVAIK GPLTTPVGGG IRSLNVALRQ QLDLYVCLRP VRYFTGVPSP 150
    VKTPEKVNMV IFRENSEDIY AGIEWPAGSP EAVKLINFLQ NEMGVKKIRF 200
    PETAGIGIKP VSKEGTSRLV RRAIQYAIDN DRDSVTLVHK GNIMKFTEGA 250
    FKDWGYEVAV KEFGAKPLDG GPWHVFENPK TGQKITIKDV IADAFLQQIL 300
    LRPAEYSVIA TLNLNGDYIS DALAAEVGGI GIAPGANLSD TVGLFEATHG 350
    TAPKYAGQDK VNPGSLILSA EMMLRYLGWK EAADLVVQGI EGAIESKTVT 400
    YDFARLMTGA KEVSTSQFGK AIIKHIL 427
    Length:427
    Mass (Da):46,636
    Last modified:May 1, 1999 - v1
    Checksum:iE23462962C26C138
    GO

    Sequence cautioni

    The sequence AAO90709.2 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti20 – 201Q → E in strain: GQ212, KoQ229, SQ217.
    Natural varianti32 – 321E → V in strain: TK-1.
    Natural varianti33 – 331V → A in strain: GQ212.
    Natural varianti52 – 521A → T in strain: GQ212, KoQ229, MAN, ME, Priscilla, SQ217.
    Natural varianti63 – 631V → A in strain: ME.
    Natural varianti87 – 871V → A in strain: SQ217.
    Natural varianti100 – 1001E → G in strain: California 76 VR 614.
    Natural varianti104 – 1041E → G in strain: 605.
    Natural varianti183 – 1831V → M in strain: Priscilla.
    Natural varianti271 – 2711G → R in strain: Bangui VR 730.
    Natural varianti306 – 3061Y → C in strain: Bangui VR 730.
    Natural varianti379 – 3791W → R in strain: El Tayeb RSA 342.
    Natural varianti415 – 4151T → A in strain: GQ212, KoQ229, SQ217.
    Natural varianti419 – 4191G → A in strain: GQ212, KoQ229, SQ217.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069035 Genomic DNA. Translation: AAC68600.1.
    AF146284 Genomic DNA. Translation: AAF16725.1.
    AF146285 Genomic DNA. Translation: AAF16726.1.
    AF146286 Genomic DNA. Translation: AAF16727.1.
    AF146287 Genomic DNA. Translation: AAF16728.1.
    AF146288 Genomic DNA. Translation: AAF16729.1.
    AF146289 Genomic DNA. Translation: AAF16730.1.
    AF146290 Genomic DNA. Translation: AAF16731.1.
    AF146291 Genomic DNA. Translation: AAF16732.1.
    AF146292 Genomic DNA. Translation: AAF16733.1.
    AF146293 Genomic DNA. Translation: AAF16734.1.
    AF146294 Genomic DNA. Translation: AAF16735.1.
    AF146295 Genomic DNA. Translation: AAF16736.1.
    AF146296 Genomic DNA. Translation: AAF16737.1.
    AF146297 Genomic DNA. Translation: AAF16738.1.
    AF146298 Genomic DNA. Translation: AAF16739.1.
    AF146299 Genomic DNA. Translation: AAF16740.1.
    AF146300 Genomic DNA. Translation: AAF16741.1.
    AF146301 Genomic DNA. Translation: AAF16742.1.
    AE016828 Genomic DNA. Translation: AAO90709.2. Different initiation.
    RefSeqiNP_820195.2. NC_002971.3.
    WP_010958068.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO90709; AAO90709; CBU_1200.
    GeneIDi1209104.
    KEGGicbu:CBU_1200.
    PATRICi17931139. VBICoxBur82552_1195.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069035 Genomic DNA. Translation: AAC68600.1 .
    AF146284 Genomic DNA. Translation: AAF16725.1 .
    AF146285 Genomic DNA. Translation: AAF16726.1 .
    AF146286 Genomic DNA. Translation: AAF16727.1 .
    AF146287 Genomic DNA. Translation: AAF16728.1 .
    AF146288 Genomic DNA. Translation: AAF16729.1 .
    AF146289 Genomic DNA. Translation: AAF16730.1 .
    AF146290 Genomic DNA. Translation: AAF16731.1 .
    AF146291 Genomic DNA. Translation: AAF16732.1 .
    AF146292 Genomic DNA. Translation: AAF16733.1 .
    AF146293 Genomic DNA. Translation: AAF16734.1 .
    AF146294 Genomic DNA. Translation: AAF16735.1 .
    AF146295 Genomic DNA. Translation: AAF16736.1 .
    AF146296 Genomic DNA. Translation: AAF16737.1 .
    AF146297 Genomic DNA. Translation: AAF16738.1 .
    AF146298 Genomic DNA. Translation: AAF16739.1 .
    AF146299 Genomic DNA. Translation: AAF16740.1 .
    AF146300 Genomic DNA. Translation: AAF16741.1 .
    AF146301 Genomic DNA. Translation: AAF16742.1 .
    AE016828 Genomic DNA. Translation: AAO90709.2 . Different initiation.
    RefSeqi NP_820195.2. NC_002971.3.
    WP_010958068.1. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q9ZH99.
    SMRi Q9ZH99. Positions 12-426.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9ZH99.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO90709 ; AAO90709 ; CBU_1200 .
    GeneIDi 1209104.
    KEGGi cbu:CBU_1200.
    PATRICi 17931139. VBICoxBur82552_1195.

    Phylogenomic databases

    KOi K00031.
    OMAi CLRPIRY.
    OrthoDBi EOG6SNDTP.

    Enzyme and pathway databases

    BioCyci CBUR227377:GJ7S-1188-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004439. Isocitrate_DH_NADP_dimer_prok.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00183. prok_nadp_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii."
      Van Nguyen S., To H., Yamaguchi T., Fukushi H., Hirai K.
      FEMS Microbiol. Lett. 175:101-106(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPTIMAL PH.
      Strain: Nine Mile I.
    2. "Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene."
      Nguyen S.V., Hirai K.
      FEMS Microbiol. Lett. 180:249-254(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1M, 27M, 307, 3M, 605, 60M, 82M, Bangui VR 730, El Tayeb RSA 342, GQ212, KoQ229, MAN, ME, Ohio 314 VR 542, Priscilla, SQ217 and TK-1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.
    4. "Proteome and antigen profiling of Coxiella burnetii developmental forms."
      Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.
      Infect. Immun. 75:290-298(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
      Strain: Nine Mile Crazy / RSA 514.

    Entry informationi

    Entry nameiIDH_COXBU
    AccessioniPrimary (citable) accession number: Q9ZH99
    Secondary accession number(s): Q7C3F6
    , Q9RG62, Q9RG63, Q9RG64, Q9RG65, Q9RG66, Q9RG67, Q9RG68, Q9RG69, Q9RG70, Q9RG71, Q9RG72
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3