Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

pH dependencei

Optimum pH is 6.5-7.7.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141NADPBy similarity
Binding sitei123 – 1231SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei129 – 1291SubstrateBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei163 – 1631SubstrateBy similarity
Sitei170 – 1701Critical for catalysisBy similarity
Sitei240 – 2401Critical for catalysisBy similarity
Metal bindingi317 – 3171Magnesium or manganeseBy similarity
Binding sitei362 – 3621NADP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei401 – 4011NADPBy similarity
Binding sitei405 – 4051NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi349 – 3557NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-1188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:icd
Ordered Locus Names:CBU_1200
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Isocitrate dehydrogenase [NADP]PRO_0000323713Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei123 – 1231PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9ZH99.

Expressioni

Developmental stagei

Detected in both the small cell variant (SCV) and in the large cell variant (LCV) stage (at protein level). LCVs are more metabolically active than SCVs.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9ZH99.
SMRiQ9ZH99. Positions 12-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00031.
OMAiFRDWGYE.
OrthoDBiEOG6SNDTP.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZH99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELTGVSIV TYQHIKVPSQ GEKITVNKAV LEVPDRPIIP FIEGDGIGID
60 70 80 90 100
IAPVMKNVVD AAVEKSYAGK RKIEWMEIYA GEKATKVYGK DNWLPDETLE
110 120 130 140 150
AIKEYQVAIK GPLTTPVGGG IRSLNVALRQ QLDLYVCLRP VRYFTGVPSP
160 170 180 190 200
VKTPEKVNMV IFRENSEDIY AGIEWPAGSP EAVKLINFLQ NEMGVKKIRF
210 220 230 240 250
PETAGIGIKP VSKEGTSRLV RRAIQYAIDN DRDSVTLVHK GNIMKFTEGA
260 270 280 290 300
FKDWGYEVAV KEFGAKPLDG GPWHVFENPK TGQKITIKDV IADAFLQQIL
310 320 330 340 350
LRPAEYSVIA TLNLNGDYIS DALAAEVGGI GIAPGANLSD TVGLFEATHG
360 370 380 390 400
TAPKYAGQDK VNPGSLILSA EMMLRYLGWK EAADLVVQGI EGAIESKTVT
410 420
YDFARLMTGA KEVSTSQFGK AIIKHIL
Length:427
Mass (Da):46,636
Last modified:May 1, 1999 - v1
Checksum:iE23462962C26C138
GO

Sequence cautioni

The sequence AAO90709.2 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti20 – 201Q → E in strain: GQ212, KoQ229, SQ217.
Natural varianti32 – 321E → V in strain: TK-1.
Natural varianti33 – 331V → A in strain: GQ212.
Natural varianti52 – 521A → T in strain: GQ212, KoQ229, MAN, ME, Priscilla, SQ217.
Natural varianti63 – 631V → A in strain: ME.
Natural varianti87 – 871V → A in strain: SQ217.
Natural varianti100 – 1001E → G in strain: California 76 VR 614.
Natural varianti104 – 1041E → G in strain: 605.
Natural varianti183 – 1831V → M in strain: Priscilla.
Natural varianti271 – 2711G → R in strain: Bangui VR 730.
Natural varianti306 – 3061Y → C in strain: Bangui VR 730.
Natural varianti379 – 3791W → R in strain: El Tayeb RSA 342.
Natural varianti415 – 4151T → A in strain: GQ212, KoQ229, SQ217.
Natural varianti419 – 4191G → A in strain: GQ212, KoQ229, SQ217.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069035 Genomic DNA. Translation: AAC68600.1.
AF146284 Genomic DNA. Translation: AAF16725.1.
AF146285 Genomic DNA. Translation: AAF16726.1.
AF146286 Genomic DNA. Translation: AAF16727.1.
AF146287 Genomic DNA. Translation: AAF16728.1.
AF146288 Genomic DNA. Translation: AAF16729.1.
AF146289 Genomic DNA. Translation: AAF16730.1.
AF146290 Genomic DNA. Translation: AAF16731.1.
AF146291 Genomic DNA. Translation: AAF16732.1.
AF146292 Genomic DNA. Translation: AAF16733.1.
AF146293 Genomic DNA. Translation: AAF16734.1.
AF146294 Genomic DNA. Translation: AAF16735.1.
AF146295 Genomic DNA. Translation: AAF16736.1.
AF146296 Genomic DNA. Translation: AAF16737.1.
AF146297 Genomic DNA. Translation: AAF16738.1.
AF146298 Genomic DNA. Translation: AAF16739.1.
AF146299 Genomic DNA. Translation: AAF16740.1.
AF146300 Genomic DNA. Translation: AAF16741.1.
AF146301 Genomic DNA. Translation: AAF16742.1.
AE016828 Genomic DNA. Translation: AAO90709.2. Different initiation.
RefSeqiNP_820195.2. NC_002971.3.
WP_010958068.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90709; AAO90709; CBU_1200.
GeneIDi1209104.
KEGGicbu:CBU_1200.
PATRICi17931139. VBICoxBur82552_1195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069035 Genomic DNA. Translation: AAC68600.1.
AF146284 Genomic DNA. Translation: AAF16725.1.
AF146285 Genomic DNA. Translation: AAF16726.1.
AF146286 Genomic DNA. Translation: AAF16727.1.
AF146287 Genomic DNA. Translation: AAF16728.1.
AF146288 Genomic DNA. Translation: AAF16729.1.
AF146289 Genomic DNA. Translation: AAF16730.1.
AF146290 Genomic DNA. Translation: AAF16731.1.
AF146291 Genomic DNA. Translation: AAF16732.1.
AF146292 Genomic DNA. Translation: AAF16733.1.
AF146293 Genomic DNA. Translation: AAF16734.1.
AF146294 Genomic DNA. Translation: AAF16735.1.
AF146295 Genomic DNA. Translation: AAF16736.1.
AF146296 Genomic DNA. Translation: AAF16737.1.
AF146297 Genomic DNA. Translation: AAF16738.1.
AF146298 Genomic DNA. Translation: AAF16739.1.
AF146299 Genomic DNA. Translation: AAF16740.1.
AF146300 Genomic DNA. Translation: AAF16741.1.
AF146301 Genomic DNA. Translation: AAF16742.1.
AE016828 Genomic DNA. Translation: AAO90709.2. Different initiation.
RefSeqiNP_820195.2. NC_002971.3.
WP_010958068.1. NC_002971.3.

3D structure databases

ProteinModelPortaliQ9ZH99.
SMRiQ9ZH99. Positions 12-426.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9ZH99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO90709; AAO90709; CBU_1200.
GeneIDi1209104.
KEGGicbu:CBU_1200.
PATRICi17931139. VBICoxBur82552_1195.

Phylogenomic databases

KOiK00031.
OMAiFRDWGYE.
OrthoDBiEOG6SNDTP.

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-1188-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004439. Isocitrate_DH_NADP_dimer_prok.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00183. prok_nadp_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii."
    Van Nguyen S., To H., Yamaguchi T., Fukushi H., Hirai K.
    FEMS Microbiol. Lett. 175:101-106(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPTIMAL PH.
    Strain: Nine Mile I.
  2. "Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene."
    Nguyen S.V., Hirai K.
    FEMS Microbiol. Lett. 180:249-254(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 1M, 27M, 307, 3M, 605, 60M, 82M, Bangui VR 730, El Tayeb RSA 342, GQ212, KoQ229, MAN, ME, Ohio 314 VR 542, Priscilla, SQ217 and TK-1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.
  4. "Proteome and antigen profiling of Coxiella burnetii developmental forms."
    Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.
    Infect. Immun. 75:290-298(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
    Strain: Nine Mile Crazy / RSA 514.

Entry informationi

Entry nameiIDH_COXBU
AccessioniPrimary (citable) accession number: Q9ZH99
Secondary accession number(s): Q7C3F6
, Q9RG62, Q9RG63, Q9RG64, Q9RG65, Q9RG66, Q9RG67, Q9RG68, Q9RG69, Q9RG70, Q9RG71, Q9RG72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 1999
Last modified: February 4, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.