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Protein

Malate synthase

Gene

aceB

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase (SSCG_00479), Malate synthase (aceB), Malate synthase (masY), Malate synthase (aceB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691Proton acceptorBy similarity
Active sitei454 – 4541Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

BRENDAi2.3.3.9. 5988.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase (EC:2.3.3.9)
Gene namesi
Name:aceB
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 541541Malate synthasePRO_0000166878Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi443255.SclaA2_010100024059.

Structurei

3D structure databases

ProteinModelPortaliQ9ZH77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZH77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASAPSRPA VVTAPAVPRQ DEVLTEAALV FLAELHRRFT PRRDELLARR
60 70 80 90 100
ARRRAEISRT GTLDFLPETA HVRADDSWRV APAPPALQDR RVEITGPADR
110 120 130 140 150
RMTVNALNSG ARVWLADFED SSAPTWENVI SGQLALSDAY HRRIDFTDER
160 170 180 190 200
SGKSYALRPD AELATVVMRP RGWHLPERHL TGPDGGVLPG ALVDFGLCFF
210 220 230 240 250
HTARRLLDLG KGPYFYLPKL ESHQEARLWN DIFLFAQEQL GIPRGTIRAT
260 270 280 290 300
VLIETITAAY EMEEILYELR EHASGLNAGR WDYLFSIIKN FRDAGPGFVL
310 320 330 340 350
PDRNAITMTA PFMRAYTELL VRTCHRRGAH AIGGMAAFIP SRRDPEINRA
360 370 380 390 400
AFEKVRADKD REAADGFDGS WVAHPDLVPV ALASFDAVLG DRPHQKDRLR
410 420 430 440 450
EEVSVTAAEL IAVGSPAARP TAAGLLNAVR VGIRYIEAWL RGTGAVAIFH
460 470 480 490 500
LMEDAATAEI SRSQIWQWIN AGVVLENGER VTPALVRTLA AGELGALRET
510 520 530 540
LGGEEYAAGR WQEAHDLLLR VALDEEYADF LTLPAYERLT G
Length:541
Mass (Da):60,012
Last modified:May 1, 1999 - v1
Checksum:i26206E19BB4B8395
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070989 Genomic DNA. Translation: AAC83648.1.
RefSeqiWP_003954464.1. NZ_DS570636.1.

Genome annotation databases

PATRICi29744904. VBIStrCla15562_1804.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070989 Genomic DNA. Translation: AAC83648.1.
RefSeqiWP_003954464.1. NZ_DS570636.1.

3D structure databases

ProteinModelPortaliQ9ZH77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443255.SclaA2_010100024059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICi29744904. VBIStrCla15562_1804.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BRENDAi2.3.3.9. 5988.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Malate synthase from Streptomyces clavuligerus NRRL3585: cloning, molecular characterization and its control by acetate."
    Chan M., Sim T.S.
    Microbiology 144:3229-3237(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiMASY_STRC2
AccessioniPrimary (citable) accession number: Q9ZH77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.