Reviewed, UniProtKB/Swiss-Prot Q9ZH77 (MASY_STRCL)

Names and origin

Protein names

Recommended name:
    Malate synthase
    EC=2.3.3.9

Gene names

Name:

aceB

Organism

Streptomyces clavuligerus

Taxonomic identifier

1901 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	Malate synthase		PRO_0000166878
Sites
	Active site	169	1	Proton acceptor By similarity
	Active site	454	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZH77-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 26206E19BB4B8395	FASTA	541	60,012
	10 20 30 40 50 60 MSASAPSRPA VVTAPAVPRQ DEVLTEAALV FLAELHRRFT PRRDELLARR ARRRAEISRT 70 80 90 100 110 120 GTLDFLPETA HVRADDSWRV APAPPALQDR RVEITGPADR RMTVNALNSG ARVWLADFED 130 140 150 160 170 180 SSAPTWENVI SGQLALSDAY HRRIDFTDER SGKSYALRPD AELATVVMRP RGWHLPERHL 190 200 210 220 230 240 TGPDGGVLPG ALVDFGLCFF HTARRLLDLG KGPYFYLPKL ESHQEARLWN DIFLFAQEQL 250 260 270 280 290 300 GIPRGTIRAT VLIETITAAY EMEEILYELR EHASGLNAGR WDYLFSIIKN FRDAGPGFVL 310 320 330 340 350 360 PDRNAITMTA PFMRAYTELL VRTCHRRGAH AIGGMAAFIP SRRDPEINRA AFEKVRADKD 370 380 390 400 410 420 REAADGFDGS WVAHPDLVPV ALASFDAVLG DRPHQKDRLR EEVSVTAAEL IAVGSPAARP 430 440 450 460 470 480 TAAGLLNAVR VGIRYIEAWL RGTGAVAIFH LMEDAATAEI SRSQIWQWIN AGVVLENGER 490 500 510 520 530 540 VTPALVRTLA AGELGALRET LGGEEYAAGR WQEAHDLLLR VALDEEYADF LTLPAYERLT G

References

[1]

"Malate synthase from Streptomyces clavuligerus NRRL3585: cloning, molecular characterization and its control by acetate." Chan M., Sim T.S. Microbiology 144:3229-3237(1998) [PubMed: 9846758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27064 / DSM 738 / IFO 13307 / JCM 4710 / NRRL 3585.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF070989 Genomic DNA. Translation: AAC83648.1.
3D structure databases
SMR	Q9ZH77. Positions 84-385, 111-521.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 229786.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_STRCL

Accession

Primary (citable) accession number: Q9ZH77

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)