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Q9ZGN0 (DDL_MYCSM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
OrganismMycobacterium smegmatis
Taxonomic identifier1772 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177843

Regions

Domain156 – 363208ATP-grasp
Nucleotide binding184 – 23956ATP By similarity

Sites

Metal binding3181Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 2 By similarity
Metal binding3321Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZGN0 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: FE7151D858C39D43

FASTA37339,232
        10         20         30         40         50         60 
MTAPNHPPGR TRVAVVYGGR SSEHAISCVS AGSILRNLDP ERFEVVAIGI TPDGSWVLTD 

        70         80         90        100        110        120 
GRPETLAITD GKLPAVTEAS GTELALPAAP NRSGQLLALG NGPGEILAAV DVVFPVLHGP 

       130        140        150        160        170        180 
YGEDGTIQGL LELAGVPYVG SGVLASAAGM DKEYTKKLLA AEGLPIGDQV VLRPGVETLD 

       190        200        210        220        230        240 
LEQRERLGLP VFVKPARGGS SIGVSRVTAW DELPAAVALA RRHDPKVIVE AAVIGRELEC 

       250        260        270        280        290        300 
GVLEFPDGRL EASTVGEIRV AGVRGREDGF YDFATKYLED AAELDVPAKV DDDVADEIRQ 

       310        320        330        340        350        360 
LAVRAFTAIG CQGLARVDFF LTDDGPVINE INTMPGFTTI SMYPRMWAAG GIDYPTLLAA 

       370 
MVDTAIARGT GLR 

« Hide

References

[1]"A temperature-sensitive mutant of Mycobacterium smegmatis which is defective in cell wall biosynthesis."
Belanger A.E., Porter J.C., Hatfull G.F.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077728 Genomic DNA. Translation: AAC99396.1.

3D structure databases

ProteinModelPortalQ9ZGN0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_MYCSM
AccessionPrimary (citable) accession number: Q9ZGN0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways