Reviewed, UniProtKB/Swiss-Prot Q9ZGM4 (KATG1_LEGPN)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Synonyms:	katB

Organism

Legionella pneumophila

Taxonomic identifier

446 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Ref.1
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Subcellular location	Cytoplasm. Ref.2
Induction	Induced during exponential growth. Ref.1
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354815
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZGM4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D96347BDAD0B719C	FASTA	721	80,500
	10 20 30 40 50 60 MDGKVGSTTT GCPVIHGGMT STGTSNTAWW PNALNLDILH QHDTKTNPME KDFNYREEVK 70 80 90 100 110 120 KLDFEALKKD LHALMTDSQA WWPADWGHYG GLMIRMSWHA AGSYRVADGR GGAGTGNQRF 130 140 150 160 170 180 APLNSWPDNV NLDKARRLLW PIKKKYGNKI SWADLIVLAG TIAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDVYW GSEQEWLGAK RYDGKSRESL ENPLAAVQMG LIYVNPEGVN GQPDPLRTAQ 250 260 270 280 290 300 DVRVTFGRMA MNDEETVALT AGGHTVGKCH GNGNAKLLGP NPEAANVEDQ GLGWINKTTR 310 320 330 340 350 360 GIGRNTVSSG IEGAWTTHPT QWDNGYFYLL LNYDWELKKS PAGAWQWEPI HIKEEDKPVD 370 380 390 400 410 420 VEDPAIRHNP IMTDADMAMK MDPVYRKIAE RFYQDPDYFA EVFARAWFKL THRDMGPKTR 430 440 450 460 470 480 YIGPDVPKED LIWQDPVPAG NRAYDIAAAK AKIAASNLTI GEMVSTAWDS ARTFRGSDKR 490 500 510 520 530 540 GGANGARIRL KPQKDWEGNE PQRLTKVLQI LEDIATDTGA SVADVIILAG NVGIEKAAKA 550 560 570 580 590 600 AGFDIIVPFA PGRGDATDDM TDAESFDVLE PLHDGYRNWL KKTYDVRPEE LMLDRTQLMG 610 620 630 640 650 660 LTAHEMTVLV GGLRVLGTNH NNTQYGVFTD RVGALTNDFF VNLTDMANVW IPSKDNLYEI 670 680 690 700 710 720 RDRKAGNIKW TATRVDLVFG SNSILRSYAE VYAQDDNKGK FIQDFVAAWT KVMNADRFDL A

References

[1]	"Legionella pneumophila catalase-peroxidases: cloning of the katB gene and studies of KatB function." Bandyopadhyay P., Steinman H.M. J. Bacteriol. 180:5369-5374(1998) [PubMed: 9765568] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION. Strain: JR32.
[2]	"Catalase-peroxidases of Legionella pneumophila: cloning of the katA gene and studies of KatA function." Bandyopadhyay P., Steinman H.M. J. Bacteriol. 182:6679-6686(2000) [PubMed: 11073912] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: JR32.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF078110 Genomic DNA. Translation: AAC64361.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_LEGPN

Accession

Primary (citable) accession number: Q9ZGM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)