##gff-version 3
Q9ZGI3	UniProtKB	Chain	1	1562	.	.	.	ID=PRO_0000436359;Note=Narbonolide/10-deoxymethynolide synthase PikA3%2C module 5	
Q9ZGI3	UniProtKB	Domain	34	464	.	.	.	Note=Ketosynthase family 3 (KS3);Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01348	
Q9ZGI3	UniProtKB	Domain	1403	1478	.	.	.	Note=Carrier;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00258	
Q9ZGI3	UniProtKB	Region	37	1475	.	.	.	Note=Module 5;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9ZGI3	UniProtKB	Region	565	866	.	.	.	Note=Acyltransferase;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9ZGI3	UniProtKB	Region	1116	1293	.	.	.	Note=Beta-ketoacyl reductase;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9ZGI3	UniProtKB	Region	1519	1548	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
Q9ZGI3	UniProtKB	Active site	209	209	.	.	.	Note=Acyl-thioester intermediate%3B for beta-ketoacyl synthase activity;Ontology_term=ECO:0000255,ECO:0000305;evidence=ECO:0000255|PROSITE-ProRule:PRU01348,ECO:0000305|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Active site	344	344	.	.	.	Note=For beta-ketoacyl synthase activity;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01348	
Q9ZGI3	UniProtKB	Active site	384	384	.	.	.	Note=For beta-ketoacyl synthase activity;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01348	
Q9ZGI3	UniProtKB	Active site	655	655	.	.	.	Note=Acyl-ester intermediate%3B for acyltransferase activity;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03133	
Q9ZGI3	UniProtKB	Active site	1263	1263	.	.	.	Note=Acyl-ester intermediate%3B for beta-ketoacyl reductase activity;Ontology_term=ECO:0000250,ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03132,ECO:0000250|UniProtKB:Q9ZGI4	
Q9ZGI3	UniProtKB	Binding site	1124	1127	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03131	
Q9ZGI3	UniProtKB	Binding site	1147	1150	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03131	
Q9ZGI3	UniProtKB	Binding site	1176	1177	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03131	
Q9ZGI3	UniProtKB	Binding site	1226	1226	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03131	
Q9ZGI3	UniProtKB	Binding site	1248	1249	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q03131	
Q9ZGI3	UniProtKB	Modified residue	1438	1438	.	.	.	Note=O-(pantetheine 4'-phosphoryl)serine;Ontology_term=ECO:0000255,ECO:0000305;evidence=ECO:0000255|PROSITE-ProRule:PRU00258,ECO:0000305|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	735	735	.	.	.	Note=Exhibits 10-fold reduced formation of the 10-deoxymethynolide macrolactone. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	747	747	.	.	.	Note=Exhibits 5-fold reduced formation of the 10-deoxymethynolide macrolactone. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	766	766	.	.	.	Note=Exhibits a reduced formation of the 10-deoxymethynolide macrolactone. E->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	768	768	.	.	.	Note=Exhibits a reduced formation of the 10-deoxymethynolide macrolactone. E->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	1133	1133	.	.	.	Note=Exhibits 14-fold reduced formation of the 10-deoxymethynolide macrolactone. R->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	1137	1137	.	.	.	Note=Exhibits 2-fold reduced formation of the 10-deoxymethynolide macrolactone. H->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Mutagenesis	1308	1308	.	.	.	Note=Exhibits 8-fold reduced formation of the 10-deoxymethynolide macrolactone. R->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24965656;Dbxref=PMID:24965656	
Q9ZGI3	UniProtKB	Helix	1544	1546	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3F5H	
Q9ZGI3	UniProtKB	Helix	1549	1557	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3F5H	
Q9ZGI3	UniProtKB	Helix	1559	1562	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3F5H