Q9ZGI2 (Q9ZGI2_STRVZ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Type I polyketide synthase PikAIV EMBL AAC69332.1

Gene names

Name:

pikAIV EMBL AAC69332.1

Organism

Streptomyces venezuelae EMBL AAC69332.1

Taxonomic identifier

54571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	1346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Transferase RuleBase RU000693
Technical term	3D-structure PDB 1MNQ PDB 1MN6 PDB 2H7Y PDB 2H7X PDB 2HFK PDB 2HFJ PDB 1MNA PDB 3F5H
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9ZGI2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3E149C8044FBE5F2	FASTA	1,346	141,914
	10 20 30 40 50 60 MTSSNEQLVD ALRASLKENE ELRKESRRRA DRRQEPMAIV GMSCRFAGGI RSPEDLWDAV 70 80 90 100 110 120 AAGKDLVSEV PEERGWDIDS LYDPVPGRKG TTYVRNAAFL DDAAGFDAAF FGISPREALA 130 140 150 160 170 180 MDPQQRQLLE ASWEVFERAG IDPASVRGTD VGVYVGCGYQ DYAPDIRVAP EGTGGYVVTG 190 200 210 220 230 240 NSSAVASGRI AYSLGLEGPA VTVDTACSSS LVALHLALKG LRNGDCSTAL VGGVAVLATP 250 260 270 280 290 300 GAFIEFSSQQ AMAADGRTKG FASAADGLAW GEGVAVLLLE RLSDARRKGH RVLAVVRGSA 310 320 330 340 350 360 INQDGASNGL TAPHGPSQQH LIRQALADAR LTSSDVDVVE GHGTGTRLGD PIEAQALLAT 370 380 390 400 410 420 YGQGRAPGQP LRLGTLKSNI GHTQAASGVA GVIKMVQALR HGVLPKTLHV DEPTDQVDWS 430 440 450 460 470 480 AGSVELLTEA VDWPERPGRL RRAGVSAFGV GGTNAHVVLE EAPAVEESPA VEPPAGGGVV 490 500 510 520 530 540 PWPVSAKTSA ALDAQIGQLA AYAEDRTDVD PAVAARALVD SRTAMEHRAV AVGDSREALR 550 560 570 580 590 600 DALRMPEGLV RGTVTDPGRV AFVFPGQGTQ WAGMGAELLD SSPEFAAAMA ECETALSPYV 610 620 630 640 650 660 DWSLEAVVRQ APSAPTLDRV DVVQPVTFAV MVSLAKVWQH HGITPEAVIG HSQGEIAAAY 670 680 690 700 710 720 VAGALTLDDA ARVVTLRSKS IAAHLAGKGG MISLALSEEA TRQRIENLHG LSIAAVNGPT 730 740 750 760 770 780 ATVVSGDPTQ IQELAQACEA DGIRARIIPV DYASHSAHVE TIENELADVL AGLSPQTPQV 790 800 810 820 830 840 PFFSTLEGTW ITEPALDGGY WYRNLRHRVG FAPAVETLAT DEGFTHFIEV SAHPVLTMTL 850 860 870 880 890 900 PDKVTGLATL RREDGGQHRL TTSLAEAWAN GLALDWASLL PATGALSPAV PDLPTYAFQH 910 920 930 940 950 960 RSYWISPAGP GEAPAHTASG REAVAETGLA WGPGAEDLDE EGRRSAVLAM VMRQAASVLR 970 980 990 1000 1010 1020 CDSPEEVPVD RPLREIGFDS LTAVDFRNRV NRLTGLQLPP TVVFQHPTPV ALAERISDEL 1030 1040 1050 1060 1070 1080 AERNWAVAEP SDHEQAEEEK AAAPAGARSG ADTGAGAGMF RALFRQAVED DRYGEFLDVL 1090 1100 1110 1120 1130 1140 AEASAFRPQF ASPEACSERL DPVLLAGGPT DRAEGRAVLV GCTGTAANGG PHEFLRLSTS 1150 1160 1170 1180 1190 1200 FQEERDFLAV PLPGYGTGTG TGTALLPADL DTALDAQARA ILRAAGDAPV VLLGHSGGAL 1210 1220 1230 1240 1250 1260 LAHELAFRLE RAHGAPPAGI VLVDPYPPGH QEPIEVWSRQ LGEGLFAGEL EPMSDARLLA 1270 1280 1290 1300 1310 1320 MGRYARFLAG PRPGRSSAPV LLVRASEPLG DWQEERGDWR AHWDLPHTVA DVPGDHFTMM 1330 1340 RDHAPAVAEA VLSWLDAIEG IEGAGK

References

[1]	"A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity." Xue Y., Zhao L., Liu H.W., Sherman D.H. Proc. Natl. Acad. Sci. U.S.A. 95:12111-12116(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15439 EMBL AAC69332.1.
[2]	"Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases." Tsai S.C., Lu H., Cane D.E., Khosla C., Stroud R.M. Biochemistry 41:12598-12606(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1049-1346.
[3]	"Structural and mechanistic insights into polyketide macrolactonization from polyketide-based affinity labels." Giraldes J.W., Akey D.L., Kittendorf J.D., Sherman D.H., Smith J.L., Fecik R.A. Nat. Chem. Biol. 2:531-536(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 1049-1346.
[4]	"Structural basis for macrolactonization by the pikromycin thioesterase." Akey D.L., Kittendorf J.D., Giraldes J.W., Fecik R.A., Sherman D.H., Smith J.L. Nat. Chem. Biol. 2:537-542(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) OF 1049-1346.
[5]	"Structural basis for binding specificity between subclasses of modular polyketide synthase docking domains." Buchholz T.J., Geders T.W., Bartley F.E., Reynolds K.A., Smith J.L., Sherman D.H. ACS Chem. Biol. 4:41-52(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF079138 Genomic DNA. Translation: AAC69332.1.

PIR

T17412.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MN6	X-ray	2.20	A/B	1049-1346	[»]
1MNA	X-ray	1.80	A/B	1049-1346	[»]
1MNQ	X-ray	2.20	A/B	1049-1346	[»]
2H7X	X-ray	1.85	A/B	1049-1346	[»]
2H7Y	X-ray	2.10	A/B	1049-1346	[»]
2HFJ	X-ray	1.95	A/B	1049-1346	[»]
2HFK	X-ray	1.79	A/B	1049-1346	[»]
3F5H	X-ray	1.75	A/B	1-37	[»]

ProteinModelPortal

Q9ZGI2.

SMR

Q9ZGI2. Positions 34-906, 1057-1339.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

Gene3D

1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.

InterPro

IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR020806. PKS_PP-bd.
IPR020802. PKS_thioesterase.
IPR001031. Thioesterase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]

Pfam

PF00698. Acyl_transf_1. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF00550. PP-binding. 1 hit.
PF00975. Thioesterase. 1 hit.
[Graphical view]

SMART

SM00827. PKS_AT. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
SM00824. PKS_TE. 1 hit.
[Graphical view]

SUPFAM

SSF47336. ACP_like. 1 hit.
SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.
SSF53901. Thiolase-like. 1 hit.

PROSITE

PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9ZGI2.

Entry information

Entry name

Q9ZGI2_STRVZ

Accession

Primary (citable) accession number: Q9ZGI2

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1999
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)