Reviewed, UniProtKB/Swiss-Prot Q9ZFV8 (MAO2_SALTY)

Names and origin

Protein names

Recommended name:
    NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Name:	maeB
Ordered Locus Names:	STM2472

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	In the N-terminal section; belongs to the malic enzymes family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	NADP-dependent malic enzyme		PRO_0000160244
Regions
	Nucleotide binding	76 – 83	8	NADP By similarity
	Region	1 – 428	428	Malic enzyme
	Region	429 – 759	331	Phosphate acetyltransferase
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	136	1	Divalent metal cation By similarity
	Metal binding	137	1	Divalent metal cation By similarity
	Binding site	162	1	NAD By similarity
	Binding site	288	1	NAD By similarity
Experimental info
	Sequence conflict	610	1	A → G in AAC78109. Ref.2
	Sequence conflict	613	1	A → P in AAC78109. Ref.2
	Sequence conflict	621	1	R → H in AAC78109. Ref.2
	Sequence conflict	626	1	E → G in AAC78109. Ref.2
	Sequence conflict	635	1	S → C in AAC78109. Ref.2
	Sequence conflict	643	1	L → P in AAC78109. Ref.2
	Sequence conflict	679	1	R → C in AAC78109. Ref.2
	Sequence conflict	754	1	A → G in AAC78109. Ref.2
	Sequence conflict	757	1	T → Q in AAC78109. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9ZFV8-1 [UniParc]. Last modified December 13, 2001. Version 2. Checksum: D5120B020158EF18	FASTA	759	82,322
	10 20 30 40 50 60 MDEQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLAAYKY 70 80 90 100 110 120 TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGIDVFDIE VDELDPDKFI 130 140 150 160 170 180 NVVAALEPTF GGINLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS TAAILNGLRV 190 200 210 220 230 240 VEKNISDVRM VVSGAGAAAI ACMNLLVALG MQKHNIVVCD SKGVIYKGRE PNMAETKAAY 250 260 270 280 290 300 AVDDSGKRTL DEVIDGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE 310 320 330 340 350 360 VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA IAELAHAEQS 370 380 390 400 410 420 EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA MDSGVATRPI ADFDAYIDKL 430 440 450 460 470 480 TEFVYKTNLF MKPIFSQARK DPKRVVLPEG EEARVLHATQ ELITLGLAKP ILIGRPSVIE 490 500 510 520 530 540 MRIQKLGLQI KAGVDFEIVN NESDPRFKEY WSEYYQIMKR RGITQEQAQR AMIGNHTAIG 550 560 570 580 590 600 AIMVQRGEAD AMICGTIGDY HEHFSVVKAV FGYRDGVHTA GAMNALLLPS GNTFIADTYV 610 620 630 640 650 660 NEDPTPEQLA EIAVMAAETV RRFGIEPKVA LLSHSNFGSS NSLSASKMRE TLERVRERAP 670 680 690 700 710 720 DLMIDGEMHG DAALVESIRN DRMPDSPLKG AANILVMPNM EAARISYNLL RVSSSEGVTV 730 740 750 GPVLMGVSKP VHVLTPIASV RRIVNMVALA VVEAQTTPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins." Kofoid E.C., Rappleye C.A., Stojiljkovic I., Roth J.R. J. Bacteriol. 181:5317-5329(1999) [PubMed: 10464203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 610-759. Strain: LT2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008811 Genomic DNA. Translation: AAL21366.1. AF093749 Genomic DNA. Translation: AAC78109.1.
RefSeq	NP_461407.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1253994.
GenomeReviews	Gene locus STM2472 in contig AE006468_GR.
KEGG	stm:STM2472.
NMPDR	fig|99287.1.peg.2388.
Organism-specific databases
StyGene	maeB. SG10658.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZFV8.
Enzyme and pathway databases
BioCyc	STYP99287:STM2472-MON.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR012188. ME_PTA. IPR016040. NAD(P)-bd. IPR002505. PTA_PTB. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF036684. ME_PTA. 1 hit.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

MAO2_SALTY

Accession

Primary (citable) accession number: Q9ZFV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 13, 2001
Last modified:	November 4, 2008
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)