Reviewed, UniProtKB/Swiss-Prot Q9ZFR5 (URED_YERPE)

Names and origin

Protein names

Recommended name:
Putative urease accessory protein ureD

Gene names

Name:	ureD
Ordered Locus Names:	YPO2671, y1243, YP_2472

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Uncertain.

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. Ref.1
Subunit structure	UreD, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity. HAMAP MF_01384
Subcellular location	Cytoplasm By similarity HAMAP MF_01384.
Sequence similarities	Belongs to the ureD family.
Caution	Could be the product of a pseudogene. This gene encodes a non-functional protein due to an extra G nucleotide which gives rise to a truncated protein. This reverts with a frequency of 10 -4 to 10 -5 on urea containing agar. This reversion does not effect virulence in mice in any detectable fashion, suggesting urease is not important in the virulence of Y.pestis (Ref.1).

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Putative urease accessory protein ureD HAMAP MF_01384		PRO_0000067621

Sequences

	Sequence		Length	Mass (Da)
	Q9ZFR5-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3D528D8890320940	FASTA	277	30,816
	10 20 30 40 50 60 MTAQSQNIVE TPSRVRAHAL GVNAPELAKY QDEPAQMRSG AVGKSGYLKL RFAKREHCSI 70 80 90 100 110 120 LAEMERRVPS LVQKALYWDE EIPELPCVTM ISTSGCILQG DRLATDVHVE AGACAHVTTQ 130 140 150 160 170 180 SATKVHMMNA NYASQIQNFI VEEGGYLEFM PDPLIPHRNS RFITDTTISI HPTATAIYSE 190 200 210 220 230 240 VLMSGRKYHH ADERFGFDVY SSRVAAQNLA GKELFVEKYV LEPKVESLDA VGVMQTFDAF 250 260 270 GNVILLTPKE HHDRILARVP AHFDIKGGDC QRRDAST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Silencing and reactivation of urease in Yersinia pestis is determined by one G residue at a specific position in the ureD gene." Sebbane F., Devalckenaere A., Foulon J., Carniel E., Simonet M. Infect. Immun. 69:170-176(2001) [PubMed: 11119503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION AS A PSEUDOGENE, REVERSION FREQUENCY, LACK OF ROLE IN VIRULENCE. Strain: 6/69M.
[2]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[3]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[4]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF095636 Genomic DNA. Translation: AAC78638.1. AL590842 Genomic DNA. No translation available. AE009952 Genomic DNA. No translation available. AE017042 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus YPO2671 in contig AE009952_GR. Gene locus YPO2671 in contig AE017042_GR. Gene locus YPO2671 in contig AL590842_GR.
KEGG	ype:YPO2671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG402559.
OMA	NGRTISH.
Family and domain databases
HAMAP	MF_01384. UreD. Divergent sequence. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED_YERPE

Accession

Primary (citable) accession number: Q9ZFR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)