Q9ZFL0 (ISPH2_BURPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2
EC=1.17.1.2

Gene names

Name:	ispH2
Synonyms:	ispH, lytB
Ordered Locus Names:	BPSS2168

Organism

Burkholderia pseudomallei (Pseudomonas pseudomallei)

Taxonomic identifier

28450 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191		PRO_0000128794
Experimental info
	Sequence conflict	297	1	V → F in AAC78334. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZFL0 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 944BC58A7CBE079B	FASTA	313	33,769
	10 20 30 40 50 60 MRVILAQPRG FCAGVVRAIE IVERALQQNG APVYVRHEIV HNRHVVENLR NKGARFVEEL 70 80 90 100 110 120 DEVPHGAVAI FSAHGVAQTV EQDAQARGLD VLDATCPLVT KVHVQGRQYV AAGRRLILIG 130 140 150 160 170 180 HAGHPEVEGT IGQIPAEVIL VQSEAEVDTL TLPADTPVAY VTQTTLSVDD TRGIIEALQR 190 200 210 220 230 240 RFTDIVGPDT RDICYATQNR QAAVRELSEQ VDVLLVVGAT NSSNSNRLRE IGTESGVPSY 250 260 270 280 290 300 LVADGSEVRA EWFANARTVG LTAGASAPEE MVEDVIAALR ALGPLEVATM SGREEKVEFK 310 LPAKLTQAVA REV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of polymyxin B-susceptible mutants of Burkholderia pseudomallei and molecular characterization of genetic loci involved in polymyxin B resistance." Burtnick M.N., Woods D.E. Antimicrob. Agents Chemother. 43:2648-2656(1999) [PubMed: 10543742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1026b.
[2]	"Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. , Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF098521 Genomic DNA. Translation: AAC78334.1. BX571966 Genomic DNA. Translation: CAH39652.1.
RefSeq	YP_112169.1. NC_006351.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3095062.
GenomeReviews	Gene locus BPSS2168 in contig BX571966_GR.
KEGG	bps:BPSS2168.
PATRIC	19272818. VBIBurPse99623_6579.
Phylogenomic databases
HOGENOM	HBG335228.
OMA	HNQGKRY.
PhylomeDB	Q9ZFL0.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	BPSE272560:BPSS2168-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH2_BURPS

Accession

Primary (citable) accession number: Q9ZFL0
Secondary accession number(s): Q63IA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program