Reviewed,
UniProtKB/Swiss-Prot Q9ZFL0 (ISPH2_BURPS)
Last modified
February 9, 2010.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 EC=1.17.1.2 | ||||||
| Gene names |
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| Organism | Burkholderia pseudomallei (Pseudomonas pseudomallei) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 28450 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group |
Protein attributes
| Sequence length | 313 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191 |
| Catalytic activity | Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 |
| Cofactor | Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191 |
| Pathway | Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191 |
| Sequence similarities | Belongs to the ispH family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Isoprene biosynthesis |
| Ligand | 3Fe-4S Iron Iron-sulfur Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic processInferred from electronic annotation. Source: HAMAP |
| Molecular function | 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activityInferred from electronic annotation. Source: HAMAP iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 313 | 313 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191 | PRO_0000128794 | |||||
Experimental info | |||||||||
| Sequence conflict | 297 | 1 | V → F in AAC78334. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation of polymyxin B-susceptible mutants of Burkholderia pseudomallei and molecular characterization of genetic loci involved in polymyxin B resistance." Burtnick M.N., Woods D.E. Antimicrob. Agents Chemother. 43:2648-2656(1999) [PubMed: 10543742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1026b. |
| [2] | "Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei." Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L. Parkhill J.Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004) [PubMed: 15377794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K96243. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF098521 Genomic DNA. Translation: AAC78334.1. BX571966 Genomic DNA. Translation: CAH39652.1. |
| RefSeq | YP_112169.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 3095062. |
| GenomeReviews | Gene locus BPSS2168 in contig BX571966_GR. |
| KEGG | bps:BPSS2168. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG335228. |
| OMA | REENIEF. |
| PhylomeDB | Q9ZFL0. |
Enzyme and pathway databases | |
| BioCyc | BPSE272560:BPSS2168-MONOMER. |
| BRENDA | 1.17.1.2. 275934. |
Family and domain databases | |
| HAMAP | MF_00191. IspH. [Tree] |
| InterPro | IPR003451. LytB. [Graphical view] |
| Pfam | PF02401. LYTB. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00216. ispH_lytB. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | ISPH2_BURPS | ||||||||
| Accession | Primary (citable) accession number: Q9ZFL0 Secondary accession number(s): Q63IA3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


