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Protein

2-dehydro-3-deoxyphosphooctonate aldolase

Gene

kdsA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate.

Pathwayi: 3-deoxy-D-manno-octulosonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Arabinose 5-phosphate isomerase KdsD (kdsD)
  2. 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA)
  3. no protein annotated in this organism
This subpathway is part of the pathway 3-deoxy-D-manno-octulosonate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate, the pathway 3-deoxy-D-manno-octulosonate biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • carbon utilization Source: PseudoCAP
  • keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: UniProtKB-HAMAP
  • lipopolysaccharide biosynthetic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.55. 5087.
UniPathwayiUPA00030.
UPA00357; UER00474.

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase (EC:2.5.1.55)
Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name:
KDO 8-P synthase
Short name:
KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase
Gene namesi
Name:kdsA
Ordered Locus Names:PA3636
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3636.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Gram-negative-bacterium-type cell wall Source: PseudoCAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001871501 – 2812-dehydro-3-deoxyphosphooctonate aldolaseAdd BLAST281

Proteomic databases

PaxDbiQ9ZFK4.
PRIDEiQ9ZFK4.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3636.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi11 – 13Combined sources3
Beta strandi15 – 17Combined sources3
Beta strandi20 – 27Combined sources8
Helixi31 – 48Combined sources18
Beta strandi52 – 58Combined sources7
Beta strandi64 – 68Combined sources5
Helixi73 – 88Combined sources16
Beta strandi92 – 95Combined sources4
Helixi99 – 101Combined sources3
Helixi102 – 108Combined sources7
Beta strandi110 – 114Combined sources5
Turni116 – 120Combined sources5
Helixi122 – 130Combined sources9
Beta strandi133 – 139Combined sources7
Helixi145 – 147Combined sources3
Helixi148 – 157Combined sources10
Beta strandi163 – 167Combined sources5
Beta strandi173 – 175Combined sources3
Helixi182 – 188Combined sources7
Beta strandi194 – 198Combined sources5
Helixi216 – 227Combined sources12
Beta strandi232 – 237Combined sources6
Helixi258 – 273Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LU0X-ray2.80A/B/C/D2-281[»]
ProteinModelPortaliQ9ZFK4.
SMRiQ9ZFK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KdsA family.Curated

Phylogenomic databases

eggNOGiENOG4105CXR. Bacteria.
COG2877. LUCA.
HOGENOMiHOG000023021.
InParanoidiQ9ZFK4.
KOiK01627.
OMAiFRGIPTM.
PhylomeDBiQ9ZFK4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00056. KDO8P_synth. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006269. KDO8P_synthase.
[Graphical view]
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01362. KDO8P_synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZFK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKIVRVGD IQIGNDLPFV LFGGMNVLES RDLAMQVCEE YVRVTEKLGI
60 70 80 90 100
PYVFKASFDK ANRSSIHSFR GPGLEEGMKI FEEIKKTFKV PVITDVHEPF
110 120 130 140 150
QAQPVAEVCD IIQLPAFLSR QTDLVVAMAR TNAVINIKKA QFLAPQEMKH
160 170 180 190 200
ILTKCEEAGN DRLILCERGS SFGYNNLVVD MLGFGIMKQF EYPVFFDVTH
210 220 230 240 250
ALQMPGGRAD SAGGRRAQVT DLAKAGLSQK LAGLFLEAHP DPEHAKCDGP
260 270 280
CALRLNKLEA FLSQLKQLDE LIKSFPAIET A
Length:281
Mass (Da):31,143
Last modified:May 1, 1999 - v1
Checksum:i7AAED3D4C0EEFA99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098791 Genomic DNA. Translation: AAD13217.1.
AE004091 Genomic DNA. Translation: AAG07024.1.
PIRiA83192.
RefSeqiNP_252326.1. NC_002516.2.
WP_003092365.1. NZ_ASJY01000590.1.

Genome annotation databases

EnsemblBacteriaiAAG07024; AAG07024; PA3636.
GeneIDi880428.
KEGGipae:PA3636.
PATRICi19841895. VBIPseAer58763_3805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098791 Genomic DNA. Translation: AAD13217.1.
AE004091 Genomic DNA. Translation: AAG07024.1.
PIRiA83192.
RefSeqiNP_252326.1. NC_002516.2.
WP_003092365.1. NZ_ASJY01000590.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LU0X-ray2.80A/B/C/D2-281[»]
ProteinModelPortaliQ9ZFK4.
SMRiQ9ZFK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3636.

Proteomic databases

PaxDbiQ9ZFK4.
PRIDEiQ9ZFK4.

Protocols and materials databases

DNASUi880428.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07024; AAG07024; PA3636.
GeneIDi880428.
KEGGipae:PA3636.
PATRICi19841895. VBIPseAer58763_3805.

Organism-specific databases

PseudoCAPiPA3636.

Phylogenomic databases

eggNOGiENOG4105CXR. Bacteria.
COG2877. LUCA.
HOGENOMiHOG000023021.
InParanoidiQ9ZFK4.
KOiK01627.
OMAiFRGIPTM.
PhylomeDBiQ9ZFK4.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00357; UER00474.
BRENDAi2.5.1.55. 5087.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00056. KDO8P_synth. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006269. KDO8P_synthase.
[Graphical view]
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01362. KDO8P_synth. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKDSA_PSEAE
AccessioniPrimary (citable) accession number: Q9ZFK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.