ID   FABI_PSEAE              Reviewed;         265 AA.
AC   Q9ZFE4;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   16-JUN-2009, entry version 62.
DE   RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH];
DE            EC=1.3.1.9;
DE   AltName: Full=NADH-dependent enoyl-ACP reductase;
GN   Name=fabI; OrderedLocusNames=PA1806;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=99395061; PubMed=10464225;
RA   Hoang T.T., Schweizer H.P.;
RT   "Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein
RT   reductase (FabI): a target for the antimicrobial triclosan and its
RT   role in acylated homoserine lactone synthesis.";
RL   J. Bacteriol. 181:5489-5497(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY: Acyl-[acyl-carrier-protein] + NAD(+) = trans-
CC       2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein (By similarity).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. FabI subfamily.
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DR   EMBL; AF104262; AAC95362.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG05195.1; -; Genomic_DNA.
DR   PIR; C83419; C83419.
DR   RefSeq; NP_250497.1; -.
DR   HSSP; P29132; 1C14.
DR   SMR; Q9ZFE4; 2-263.
DR   GeneID; 878311; -.
DR   GenomeReviews; AE004091_GR; PA1806.
DR   KEGG; pae:PA1806; -.
DR   NMPDR; fig|208964.1.peg.1807; -.
DR   PseudoCAP; PA1806; -.
DR   HOGENOM; Q9ZFE4; -.
DR   OMA; Q9ZFE4; LVHCLAF.
DR   BioCyc; PAER208964:PA1806-MON; -.
DR   BRENDA; 1.3.1.9; 354.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NAD...; IEA:EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   PANTHER; PTHR19410:SF12; Enoyl-ACP_rdct; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome;
KW   Fatty acid biosynthesis; Lipid synthesis; Membrane; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    265       Enoyl-[acyl-carrier-protein] reductase
FT                                [NADH].
FT                                /FTId=PRO_0000054904.
FT   NP_BIND      10     36       NAD (By similarity).
FT   ACT_SITE    159    159       Proton acceptor (By similarity).
SQ   SEQUENCE   265 AA;  28006 MW;  3E7A946886DFB293 CRC64;
     MGFLTGKRAL IVGVASKLSI ASGIAAAMHR EGAELAFTYQ NDKLRGRVEE FASGWGSRPE
     LCFPCDVADD SQIEAVFAAL GKHWDGLDII VHSVGFAPGD QLDGDFTAVT TREGFRIAHD
     ISAYSFIALA KAGREMMKGR NGSLLTLSYL GAERTMPNYN VMGMAKASLE AGVRYLAGSL
     GAEGTRVNAV SAGPIRTLAA SGIKSFRKML AANERQTPLR RNVTIEEVGN AGAFLCSDLA
     SGISGEILYV DGGFNTTAMG PLDDD
//