SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9ZFE4

- FABI_PSEAE

UniProt

Q9ZFE4 - FABI_PSEAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Gene
fabI, PA1806
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen By similarity
Binding sitei40 – 401NAD By similarity
Binding sitei94 – 941NAD; via carbonyl oxygen By similarity
Binding sitei97 – 971Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei149 – 1491Proton acceptor By similarity
Active sitei159 – 1591Proton acceptor By similarity
Binding sitei166 – 1661NAD By similarity
Sitei204 – 2041Involved in acyl-ACP binding By similarity
Sitei207 – 2071Involved in acyl-ACP binding By similarity
Sitei208 – 2081Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD By similarity
Nucleotide bindingi66 – 672NAD By similarity
Nucleotide bindingi195 – 1995NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
  2. enoyl-[acyl-carrier-protein] reductase activity Source: PseudoCAP

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:PA1806
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA1806.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
PRO_0000054904Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi208964.PA1806.

Structurei

3D structure databases

ProteinModelPortaliQ9ZFE4.
SMRiQ9ZFE4. Positions 2-259.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiIAKRCAP.
OrthoDBiEOG6HF644.
PhylomeDBiQ9ZFE4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Q9ZFE4-1 [UniParc]FASTAAdd to Basket

« Hide

MGFLTGKRAL IVGVASKLSI ASGIAAAMHR EGAELAFTYQ NDKLRGRVEE    50
FASGWGSRPE LCFPCDVADD SQIEAVFAAL GKHWDGLDII VHSVGFAPGD 100
QLDGDFTAVT TREGFRIAHD ISAYSFIALA KAGREMMKGR NGSLLTLSYL 150
GAERTMPNYN VMGMAKASLE AGVRYLAGSL GAEGTRVNAV SAGPIRTLAA 200
SGIKSFRKML AANERQTPLR RNVTIEEVGN AGAFLCSDLA SGISGEILYV 250
DGGFNTTAMG PLDDD 265
Length:265
Mass (Da):28,006
Last modified:May 1, 1999 - v1
Checksum:i3E7A946886DFB293
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF104262 Genomic DNA. Translation: AAC95362.1.
AE004091 Genomic DNA. Translation: AAG05195.1.
PIRiC83419.
RefSeqiNP_250497.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG05195; AAG05195; PA1806.
GeneIDi878311.
KEGGipae:PA1806.
PATRICi19837991. VBIPseAer58763_1876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF104262 Genomic DNA. Translation: AAC95362.1 .
AE004091 Genomic DNA. Translation: AAG05195.1 .
PIRi C83419.
RefSeqi NP_250497.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q9ZFE4.
SMRi Q9ZFE4. Positions 2-259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA1806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG05195 ; AAG05195 ; PA1806 .
GeneIDi 878311.
KEGGi pae:PA1806.
PATRICi 19837991. VBIPseAer58763_1876.

Organism-specific databases

PseudoCAPi PA1806.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi IAKRCAP.
OrthoDBi EOG6HF644.
PhylomeDBi Q9ZFE4.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis."
    Hoang T.T., Schweizer H.P.
    J. Bacteriol. 181:5489-5497(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiFABI_PSEAE
AccessioniPrimary (citable) accession number: Q9ZFE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi