Reviewed, UniProtKB/Swiss-Prot Q9ZFE4 (FABI_PSEAE)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	PA1806

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054904
Regions
	Nucleotide binding	10 – 36	27	NAD By similarity
Sites
	Active site	159	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZFE4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3E7A946886DFB293	FASTA	265	28,006
	10 20 30 40 50 60 MGFLTGKRAL IVGVASKLSI ASGIAAAMHR EGAELAFTYQ NDKLRGRVEE FASGWGSRPE 70 80 90 100 110 120 LCFPCDVADD SQIEAVFAAL GKHWDGLDII VHSVGFAPGD QLDGDFTAVT TREGFRIAHD 130 140 150 160 170 180 ISAYSFIALA KAGREMMKGR NGSLLTLSYL GAERTMPNYN VMGMAKASLE AGVRYLAGSL 190 200 210 220 230 240 GAEGTRVNAV SAGPIRTLAA SGIKSFRKML AANERQTPLR RNVTIEEVGN AGAFLCSDLA 250 260 SGISGEILYV DGGFNTTAMG PLDDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis." Hoang T.T., Schweizer H.P. J. Bacteriol. 181:5489-5497(1999) [PubMed: 10464225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF104262 Genomic DNA. Translation: AAC95362.1. AE004091 Genomic DNA. Translation: AAG05195.1.
PIR	C83419.
RefSeq	NP_250497.1.
3D structure databases
HSSP	HSSP built from PDB template 1C14 based on UniProtKB P29132.
SMR	Q9ZFE4. Positions 2-263.
ModBase	Search...
Genome annotation databases
GeneID	878311.
GenomeReviews	Gene locus PA1806 in contig AE004091_GR.
KEGG	pae:PA1806.
NMPDR	fig|208964.1.peg.1807.
Organism-specific databases
PseudoCAP	PA1806.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZFE4.
OMA	Q9ZFE4. LVHCLAF.
Enzyme and pathway databases
BioCyc	PAER208964:PA1806-MON.
BRENDA	1.3.1.9. 354.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_PSEAE

Accession

Primary (citable) accession number: Q9ZFE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)