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Q9ZFE4

- FABI_PSEAE

UniProt

Q9ZFE4 - FABI_PSEAE

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
    Binding sitei40 – 401NADBy similarity
    Binding sitei94 – 941NAD; via carbonyl oxygenBy similarity
    Binding sitei97 – 971Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Active sitei149 – 1491Proton acceptorBy similarity
    Active sitei159 – 1591Proton acceptorBy similarity
    Binding sitei166 – 1661NADBy similarity
    Sitei204 – 2041Involved in acyl-ACP bindingBy similarity
    Sitei207 – 2071Involved in acyl-ACP bindingBy similarity
    Sitei208 – 2081Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NADBy similarity
    Nucleotide bindingi66 – 672NADBy similarity
    Nucleotide bindingi195 – 1995NADBy similarity

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
    2. enoyl-[acyl-carrier-protein] reductase activity Source: PseudoCAP

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB
    2. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:PA1806
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA1806.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 265265Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054904Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi208964.PA1806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZFE4.
    SMRiQ9ZFE4. Positions 2-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiIAKRCAP.
    OrthoDBiEOG6HF644.
    PhylomeDBiQ9ZFE4.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    Q9ZFE4-1 [UniParc]FASTAAdd to Basket

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    MGFLTGKRAL IVGVASKLSI ASGIAAAMHR EGAELAFTYQ NDKLRGRVEE    50
    FASGWGSRPE LCFPCDVADD SQIEAVFAAL GKHWDGLDII VHSVGFAPGD 100
    QLDGDFTAVT TREGFRIAHD ISAYSFIALA KAGREMMKGR NGSLLTLSYL 150
    GAERTMPNYN VMGMAKASLE AGVRYLAGSL GAEGTRVNAV SAGPIRTLAA 200
    SGIKSFRKML AANERQTPLR RNVTIEEVGN AGAFLCSDLA SGISGEILYV 250
    DGGFNTTAMG PLDDD 265
    Length:265
    Mass (Da):28,006
    Last modified:May 1, 1999 - v1
    Checksum:i3E7A946886DFB293
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104262 Genomic DNA. Translation: AAC95362.1.
    AE004091 Genomic DNA. Translation: AAG05195.1.
    PIRiC83419.
    RefSeqiNP_250497.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG05195; AAG05195; PA1806.
    GeneIDi878311.
    KEGGipae:PA1806.
    PATRICi19837991. VBIPseAer58763_1876.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104262 Genomic DNA. Translation: AAC95362.1 .
    AE004091 Genomic DNA. Translation: AAG05195.1 .
    PIRi C83419.
    RefSeqi NP_250497.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9ZFE4.
    SMRi Q9ZFE4. Positions 2-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA1806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG05195 ; AAG05195 ; PA1806 .
    GeneIDi 878311.
    KEGGi pae:PA1806.
    PATRICi 19837991. VBIPseAer58763_1876.

    Organism-specific databases

    PseudoCAPi PA1806.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi IAKRCAP.
    OrthoDBi EOG6HF644.
    PhylomeDBi Q9ZFE4.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): a target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis."
      Hoang T.T., Schweizer H.P.
      J. Bacteriol. 181:5489-5497(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiFABI_PSEAE
    AccessioniPrimary (citable) accession number: Q9ZFE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3