Q9ZF44 (PUR2_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	LL1513
ORF Names:	L153005

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAK05611.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151455
Regions
	Domain	108 – 309	202	ATP-grasp
	Nucleotide binding	134 – 190	57	ATP By similarity
Sites
	Metal binding	279	1	Magnesium or manganese By similarity
	Metal binding	281	1	Magnesium or manganese By similarity
Experimental info
	Sequence conflict	46	1	D → H in CAA04374. Ref.1
	Sequence conflict	215	1	G → E in CAA04374. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZF44 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: EAAD0D69997E1C02	FASTA	412	44,289
	10 20 30 40 50 60 MKILVIGSGG REHALAKKFM ESPQVEEVFV APGNSGMEKD GIQIVDISEL SNDKLVKFAQ 70 80 90 100 110 120 NQNIGLTFVG PETALMNGVV DAFIKAELPI FGPNKMAAEL EGSKDFAKSI MKKYGVPTAD 130 140 150 160 170 180 YATFDSLEPA LAYLDEKGVP LVIKADGLAA GKGVTVAFDI ETAKSALADI FSGSQGKVVI 190 200 210 220 230 240 EEFLDGEEFS LFSFIHDGKI YPMPIAQDHK RAFDGDKGPN TGGMGAYSPV LHISKEVVNE 250 260 270 280 290 300 ALEKVVKPTV AGMIEEGKSF TGVLYAGLIL TEDGVKTIEF NARFGDPETQ VVLPRLKSDL 310 320 330 340 350 360 AQAIIDILAG NEPTLEWLES GVTLGVVVAA EGYPSQAKLG LILPEIPEGL NVYYAGVSKN 370 380 390 400 410 ENNQLISSGG RVYLVSETGE DVKSTQKLLY EKLDKLENDG FFYRHDIGSR AI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the Lactococcus lactis purDEK genes, required for growth in milk." Nilsson D., Kilstrup M. Appl. Environ. Microbiol. 64:4321-4327(1998) [PubMed: 9797284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CHCC373.
[2]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000883 Genomic DNA. Translation: CAA04374.1. AE005176 Genomic DNA. Translation: AAK05611.1. Different initiation.
PIR	A86814.
RefSeq	NP_267669.1. NC_002662.1.
3D structure databases
ProteinModelPortal	Q9ZF44.
SMR	Q9ZF44. Positions 1-412.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1115170.
GenomeReviews	Gene locus LL1513 in contig AE005176_GR.
KEGG	lla:L153005.
NMPDR	fig|272623.1.peg.1554.
PATRIC	22295432. VBILacLac136773_1623.
Phylogenomic databases
HOGENOM	HBG404060.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	LLAC272623:L153005-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LACLA

Accession

Primary (citable) accession number: Q9ZF44

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program