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Q9ZF44 (PUR2_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:LL1513
ORF Names:L153005
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAK05611.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151455

Regions

Domain108 – 309202ATP-grasp
Nucleotide binding134 – 19057ATP By similarity

Sites

Metal binding2791Magnesium or manganese By similarity
Metal binding2811Magnesium or manganese By similarity

Experimental info

Sequence conflict461D → H in CAA04374. Ref.1
Sequence conflict2151G → E in CAA04374. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9ZF44 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: EAAD0D69997E1C02

FASTA41244,289
        10         20         30         40         50         60 
MKILVIGSGG REHALAKKFM ESPQVEEVFV APGNSGMEKD GIQIVDISEL SNDKLVKFAQ 

        70         80         90        100        110        120 
NQNIGLTFVG PETALMNGVV DAFIKAELPI FGPNKMAAEL EGSKDFAKSI MKKYGVPTAD 

       130        140        150        160        170        180 
YATFDSLEPA LAYLDEKGVP LVIKADGLAA GKGVTVAFDI ETAKSALADI FSGSQGKVVI 

       190        200        210        220        230        240 
EEFLDGEEFS LFSFIHDGKI YPMPIAQDHK RAFDGDKGPN TGGMGAYSPV LHISKEVVNE 

       250        260        270        280        290        300 
ALEKVVKPTV AGMIEEGKSF TGVLYAGLIL TEDGVKTIEF NARFGDPETQ VVLPRLKSDL 

       310        320        330        340        350        360 
AQAIIDILAG NEPTLEWLES GVTLGVVVAA EGYPSQAKLG LILPEIPEGL NVYYAGVSKN 

       370        380        390        400        410 
ENNQLISSGG RVYLVSETGE DVKSTQKLLY EKLDKLENDG FFYRHDIGSR AI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of the Lactococcus lactis purDEK genes, required for growth in milk."
Nilsson D., Kilstrup M.
Appl. Environ. Microbiol. 64:4321-4327(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CHCC373.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ000883 Genomic DNA. Translation: CAA04374.1.
AE005176 Genomic DNA. Translation: AAK05611.1. Different initiation.
PIRA86814.
RefSeqNP_267669.1. NC_002662.1.

3D structure databases

ProteinModelPortalQ9ZF44.
SMRQ9ZF44. Positions 1-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272623.L153005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05611; AAK05611; L153005.
GeneID1115170.
KEGGlla:L153005.
PATRIC22295432. VBILacLac136773_1623.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1610-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_LACLA
AccessionPrimary (citable) accession number: Q9ZF44
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 27, 2001
Last modified: February 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways