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Q9ZF44

- PUR2_LACLA

UniProt

Q9ZF44 - PUR2_LACLA

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Protein
Phosphoribosylamine--glycine ligase
Gene
purD, LL1513, L153005
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi279 – 2791Magnesium or manganese By similarity
Metal bindingi281 – 2811Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 19057ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1610-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene namesi
Name:purD
Ordered Locus Names:LL1513
ORF Names:L153005
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Phosphoribosylamine--glycine ligaseUniRule annotation
PRO_0000151455Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272623.L153005.

Structurei

3D structure databases

ProteinModelPortaliQ9ZF44.
SMRiQ9ZF44. Positions 1-412.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 309202ATP-grasp
Add
BLAST

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
KOiK01945.
OMAiCIAGLKN.
OrthoDBiEOG69SKD1.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZF44-1 [UniParc]FASTAAdd to Basket

« Hide

MKILVIGSGG REHALAKKFM ESPQVEEVFV APGNSGMEKD GIQIVDISEL    50
SNDKLVKFAQ NQNIGLTFVG PETALMNGVV DAFIKAELPI FGPNKMAAEL 100
EGSKDFAKSI MKKYGVPTAD YATFDSLEPA LAYLDEKGVP LVIKADGLAA 150
GKGVTVAFDI ETAKSALADI FSGSQGKVVI EEFLDGEEFS LFSFIHDGKI 200
YPMPIAQDHK RAFDGDKGPN TGGMGAYSPV LHISKEVVNE ALEKVVKPTV 250
AGMIEEGKSF TGVLYAGLIL TEDGVKTIEF NARFGDPETQ VVLPRLKSDL 300
AQAIIDILAG NEPTLEWLES GVTLGVVVAA EGYPSQAKLG LILPEIPEGL 350
NVYYAGVSKN ENNQLISSGG RVYLVSETGE DVKSTQKLLY EKLDKLENDG 400
FFYRHDIGSR AI 412
Length:412
Mass (Da):44,289
Last modified:April 27, 2001 - v2
Checksum:iEAAD0D69997E1C02
GO

Sequence cautioni

The sequence AAK05611.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461D → H in CAA04374. 1 Publication
Sequence conflicti215 – 2151G → E in CAA04374. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000883 Genomic DNA. Translation: CAA04374.1.
AE005176 Genomic DNA. Translation: AAK05611.1. Different initiation.
PIRiA86814.
RefSeqiNP_267669.1. NC_002662.1.
WP_003130065.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05611; AAK05611; L153005.
GeneIDi1115170.
KEGGilla:L153005.
PATRICi22295432. VBILacLac136773_1623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ000883 Genomic DNA. Translation: CAA04374.1 .
AE005176 Genomic DNA. Translation: AAK05611.1 . Different initiation.
PIRi A86814.
RefSeqi NP_267669.1. NC_002662.1.
WP_003130065.1. NC_002662.1.

3D structure databases

ProteinModelPortali Q9ZF44.
SMRi Q9ZF44. Positions 1-412.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272623.L153005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK05611 ; AAK05611 ; L153005 .
GeneIDi 1115170.
KEGGi lla:L153005.
PATRICi 22295432. VBILacLac136773_1623.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033463.
KOi K01945.
OMAi CIAGLKN.
OrthoDBi EOG69SKD1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci LLAC272623:GHSH-1610-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the Lactococcus lactis purDEK genes, required for growth in milk."
    Nilsson D., Kilstrup M.
    Appl. Environ. Microbiol. 64:4321-4327(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CHCC373.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiPUR2_LACLA
AccessioniPrimary (citable) accession number: Q9ZF44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 27, 2001
Last modified: September 3, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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