Q9ZF13 (Q9ZF13_THEFU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-mannanase EMBL CAA06924.1
EC=3.2.1.78 EMBL CAA06924.1

Gene names

Name:

man EMBL CAA06924.1

Organism

Thermomonospora fusca EMBL CAA06924.1

Taxonomic identifier

2021 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

Protein attributes

Sequence length	279 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL CAA06924.1 Hydrolase
Technical term	3D-structure PDB 2MAN PDB 3MAN PDB 1BQC
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	257 – 258	2	Mannose binding PDB 2MAN PDB 3MAN
Sites
	Binding site	28	1	Beta-D-mannose PDB 2MAN PDB 3MAN
	Binding site	57	1	Beta-D-mannose PDB 2MAN PDB 3MAN
	Site	125	1	Important for catalytic activity
	Site	126	1	Important for catalytic activity
	Site	194	1	Important for catalytic activity
	Site	196	1	Important for catalytic activity
	Site	223	1	Important for catalytic activity
Experimental info
	Non-terminal residue	1	1	EMBL CAA06924.1
	Non-terminal residue	279	1	EMBL CAA06924.1

Sequences

	Sequence		Length	Mass (Da)
	Q9ZF13 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 98A2850FAE013DB0	FASTA	279	30,657
	10 20 30 40 50 60 GLHVKNGRLY EANGQEFIIR GVSHPHNWYP QHTQAFADIK SHGANTVRVV LSNGVRWSKN 70 80 90 100 110 120 GPSDVANVIS LCKQNRLICM LEVHDTTGYG EQSGASTLDQ AVDYWIELKS VLQGEEDYVL 130 140 150 160 170 180 INIGNEPYGN DSATVAAGAW DTSAAIQRLR AAGFEHTLVV DAPNWGQDWT NTMRNNADQV 190 200 210 220 230 240 YASDPTGNTV FSIHMYGVYS QASTITSYLE HFVNAGLPLI IGEFGHDHSD GNPDEDTIMA 250 260 270 EAERLKLGYI GWSWSGNGGG VEYLDMVYNF DGDNLSPWG

References

[1]	"Crystallization and preliminary crystallographic analysis of two beta-mannanase isoforms from Thermomonospora fusca KW3." Hilge M., Gloor S., Winterhalter K., Piontek K. Acta Crystallogr. 52:1224-1225(1996) Cited for: NUCLEOTIDE SEQUENCE. Strain: KW3 EMBL CAA06924.1.
[2]	"High-resolution native and complex structures of thermostable beta-mannanase from Thermomonospora fusca - substrate specificity in glycosyl hydrolase family 5." Hilge M., Gloor S.M., Rypniewski W., Sauer O., Heightman T.D., Zimmermann W., Winterhalter K., Piontek K. Structure 6:1433-1444(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: KW3 EMBL CAA06924.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ006227 Genomic DNA. Translation: CAA06924.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BQC	X-ray	1.50	A	1-279	[»]
2MAN	X-ray	1.90	A	1-279	[»]
3MAN	X-ray	1.60	A	1-279	[»]

ProteinModelPortal

Q9ZF13.

ModBase

Search...

Protein family/group databases

CAZy

GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.20.20.80. 1 hit.

InterPro

IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Pfam

PF00150. Cellulase. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9ZF13.

Entry information

Entry name

Q9ZF13_THEFU

Accession

Primary (citable) accession number: Q9ZF13

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1999
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)