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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:MAP_2445
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393681 – 165Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST165

Interactioni

Protein-protein interaction databases

STRINGi262316.MAP2445.

Structurei

3D structure databases

ProteinModelPortaliQ9ZET8.
SMRiQ9ZET8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K85. Bacteria.
COG0350. LUCA.
KOiK00567.
OMAiNPKSCRA.
OrthoDBiPOG091H02CY.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR036217. MethylDNA_cys_MeTrfase_DNAb.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR036631. MGMT_N_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZET8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEYRTVDSP IGLLTLAGRD PVLTNLRMVD QTYEPSRTGW TENPRAFAGA
60 70 80 90 100
VEQLGAYFAG ELTEFDIELD LRGSEFQRRV WRALQTIPYG ETRSYGEIAE
110 120 130 140 150
QIGAPGAARA VGLANGHNPI AIVVPCHRVI GASGKLTGYG GGLDRKQTLL
160
ALERRHSPAS LTLFD
Length:165
Mass (Da):17,998
Last modified:May 1, 1999 - v1
Checksum:iD39A89116861E796
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011838 Genomic DNA. Translation: CAA09802.1.
AE016958 Genomic DNA. Translation: AAS04762.1.
RefSeqiWP_010949605.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS04762; AAS04762; MAP_2445.
GeneIDi2718320.
KEGGimpa:MAP_2445.

Similar proteinsi

Entry informationi

Entry nameiOGT_MYCPA
AccessioniPrimary (citable) accession number: Q9ZET8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: February 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome