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Q9ZEM7

- ALF_STRGB

UniProt

Q9ZEM7 - ALF_STRGB

Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Streptomyces galbus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    pH dependencei

    Optimum pH is 7.5.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Glyceraldehyde 3-phosphateBy similarity
    Active sitei95 – 951Proton donorBy similarity
    Metal bindingi96 – 961Zinc 1; catalyticBy similarity
    Metal bindingi131 – 1311Zinc 2By similarity
    Metal bindingi161 – 1611Zinc 2By similarity
    Metal bindingi212 – 2121Zinc 1; catalyticBy similarity
    Binding sitei213 – 2131Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi249 – 2491Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fba
    Synonyms:fda
    OrganismiStreptomyces galbus
    Taxonomic identifieri33898 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Fructose-bisphosphate aldolasePRO_0000178744Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ZEM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni250 – 2523Dihydroxyacetone phosphate bindingBy similarity
    Regioni271 – 2744Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ZEM7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIATPEVYN EMLDRAKAGK FAYPAINVTS SQTLNAALRG FAEAESDGIV    50
    QISTGGAEFL GGQYSKDMVT GAVALAEFAH IIAEKYPVNI ALHTDHCPKD 100
    KLDGYVRPLL ALSKKRVEAG LGPLFQSHMW DGSAEPLADN LAIAQELLET 150
    ARAAQIILEV EITPTGGEED GVSHEINDSL YTTVDDAIRT AEALGLGEKG 200
    RYLLAASFGN VHGVYKPGNV VLRPELLKEL NEGVAARFGK ESPFDFVFHG 250
    GSGSSEEEIR TALENGVVKM NLDTDTQYAF TRPVAGHMFA NYDGVLKVDG 300
    EVGNKKAYDP RTWGKLAEAS MAARVVEATQ HLRSAGNKIK 340
    Length:340
    Mass (Da):36,550
    Last modified:August 14, 2001 - v2
    Checksum:i8DB47E14F8B8E9F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131707 Genomic DNA. Translation: CAA10483.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131707 Genomic DNA. Translation: CAA10483.2 .

    3D structure databases

    ProteinModelPortali Q9ZEM7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S. galbus fructose-1,6-bisphosphate aldolase is a member of the class II aldolases."
      Wehmeier U.F.
      FEMS Microbiol. Lett. 197:53-58(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: ATCC 14077 / CBS 700.72 / DSM 40480 / NBRC 13399 / VKM Ac-160.

    Entry informationi

    Entry nameiALF_STRGB
    AccessioniPrimary (citable) accession number: Q9ZEM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3