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Q9ZEM7

- ALF_STRGB

UniProt

Q9ZEM7 - ALF_STRGB

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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Streptomyces galbus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

pH dependencei

Optimum pH is 7.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Glyceraldehyde 3-phosphateBy similarity
Active sitei95 – 951Proton donorBy similarity
Metal bindingi96 – 961Zinc 1; catalyticBy similarity
Metal bindingi131 – 1311Zinc 2By similarity
Metal bindingi161 – 1611Zinc 2By similarity
Metal bindingi212 – 2121Zinc 1; catalyticBy similarity
Binding sitei213 – 2131Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi249 – 2491Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:fda
OrganismiStreptomyces galbus
Taxonomic identifieri33898 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Fructose-bisphosphate aldolasePRO_0000178744Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9ZEM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni250 – 2523Dihydroxyacetone phosphate bindingBy similarity
Regioni271 – 2744Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZEM7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIATPEVYN EMLDRAKAGK FAYPAINVTS SQTLNAALRG FAEAESDGIV
60 70 80 90 100
QISTGGAEFL GGQYSKDMVT GAVALAEFAH IIAEKYPVNI ALHTDHCPKD
110 120 130 140 150
KLDGYVRPLL ALSKKRVEAG LGPLFQSHMW DGSAEPLADN LAIAQELLET
160 170 180 190 200
ARAAQIILEV EITPTGGEED GVSHEINDSL YTTVDDAIRT AEALGLGEKG
210 220 230 240 250
RYLLAASFGN VHGVYKPGNV VLRPELLKEL NEGVAARFGK ESPFDFVFHG
260 270 280 290 300
GSGSSEEEIR TALENGVVKM NLDTDTQYAF TRPVAGHMFA NYDGVLKVDG
310 320 330 340
EVGNKKAYDP RTWGKLAEAS MAARVVEATQ HLRSAGNKIK
Length:340
Mass (Da):36,550
Last modified:August 14, 2001 - v2
Checksum:i8DB47E14F8B8E9F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131707 Genomic DNA. Translation: CAA10483.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131707 Genomic DNA. Translation: CAA10483.2 .

3D structure databases

ProteinModelPortali Q9ZEM7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S. galbus fructose-1,6-bisphosphate aldolase is a member of the class II aldolases."
    Wehmeier U.F.
    FEMS Microbiol. Lett. 197:53-58(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 14077 / CBS 700.72 / DSM 40480 / NBRC 13399 / VKM Ac-160.

Entry informationi

Entry nameiALF_STRGB
AccessioniPrimary (citable) accession number: Q9ZEM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 14, 2001
Last modified: November 26, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3