Reviewed, UniProtKB/Swiss-Prot Q9ZED5 (LPXA_RICPR)

Names and origin

Protein names

Recommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
      Short name=UDP-N-acetylglucosamine acyltransferase
    EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	RP007

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_0000188065

Sequences

	Sequence		Length	Mass (Da)
	Q9ZED5-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 9FA749DC2B5BCE61	FASTA	264	28,471
	10 20 30 40 50 60 MSNSNIHTTA IIAEGAKFGK NVKVGPYCII GPEVVLHDNV ELKSHVVIDG ITEIGENTVI 70 80 90 100 110 120 YPFASIGQPP QILKYANERS STIIGSNNTI REYVTVQAGS KSGGMITRVG NNNLFMVGVH 130 140 150 160 170 180 IGHDCKIGNN LVFANYVSLA GHIKVGDYAI IGGLSAVHQY TRIGEYSMIG GLSPVGADVI 190 200 210 220 230 240 PFGLVSSKRA VLEGLNLIGM NRKGFDKADS LTALNAVEEI FLGEGNFVDR IKQVAEKYKN 250 260 NSIVTQIIDF LNQDSSRAFC HFKK

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14480.1.
PIR	A71708.
RefSeq	NP_220403.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883506.
GenomeReviews	Gene locus RP007 in contig AJ235269_GR.
KEGG	rpr:RP007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZED5.
OMA	DLKYQGE
Enzyme and pathway databases
BioCyc	RPRO272947:RP007-MON.
BRENDA	2.3.1.129. 141977.
Family and domain databases
HAMAP	MF_00387. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 8 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_RICPR

Accession

Primary (citable) accession number: Q9ZED5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)