Q9ZED3 (LPXD_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	RP009

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059698
Sites
	Active site	253	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZED3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 51616B72A57D9150	FASTA	346	37,243
	10 20 30 40 50 60 MVSSNFYKNL GPRKLTAIVD FLHDIIEPTK IYEDIDIYDI KILQEASPND ISFLSNPKYS 70 80 90 100 110 120 EFLKTTKAAA CIVPKNFTEE VNQNTVLIHA ENSYFAYSKL IDFFYAPIKS YSTKIMKSAI 130 140 150 160 170 180 IADSATIGKN CYIGHNVVIE DDVIIGDNSI IDAGTFIGRG VNIGKNARIE QHVSINYAII 190 200 210 220 230 240 GDDVVILVGA KIGQDGFGFS TEKGVHHKIF HIGIVKIGNN VEIGSNTTID RGALQDTIIE 250 260 270 280 290 300 DLCRIDNLVQ IGHGVKIGKG SIIVAQAGIA GSSAIGKYCA LGGQVGIAGH LNIGDGTQVA 310 320 330 340 AQGGVAQNIE EGKIVGGSPA VPIMDWHRQS IIMKQLVKTS NSKLKK

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14482.1.
PIR	C71708.
RefSeq	NP_220405.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZED3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883512.
GenomeReviews	Gene locus RP009 in contig AJ235269_GR.
KEGG	rpr:RP009.
PATRIC	17900746. VBIRicPro72556_0009.
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	RPRO272947:RP009-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RICPR

Accession

Primary (citable) accession number: Q9ZED3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program