Q9ZED2 (DUS_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-dihydrouridine synthase
EC=1.-.-.-

Gene names

Name:	dus
Ordered Locus Names:	RP011

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family.
Sequence caution	The sequence CAA14483.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Probable tRNA-dihydrouridine synthase		PRO_0000162141

Sequences

	Sequence		Length	Mass (Da)
	Q9ZED2 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: AAE4B4D4A87555CC	FASTA	327	35,789
	10 20 30 40 50 60 MIKIGNIELS SNVILAPMSD VTDLEFRKLV KRFGAGLVVS EMIASRAMIM KSRQSMQKCA 70 80 90 100 110 120 IMHDDPTSAC VQLAGCEPDV IAEAAKMNED MGAKIIDLNF GCPAKKVVGG YAGSALMRDE 130 140 150 160 170 180 QLAAKIFEAT VKAVKIPVTV KMRIGWDDNT RNAPTLAKIA ANSGVQMVTV HGRTRCQFYS 190 200 210 220 230 240 GNANWDFIRT VKEAVKIPVI ANGDITNFAK AKEALQRSGA DGIMVGRGVY GKPWLISQIA 250 260 270 280 290 300 YYLKTGKEKP APSIAEQLDI ITKHYDAIID YYGKSVGVPI ARKHIIWYSN GLPSSAEFRC 310 320 TVNLMKDPIA VKEKIAEFYM SVMDANK

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14483.1. Different initiation.
PIR	D71708.
RefSeq	NP_220406.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZED2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883511.
GenomeReviews	Gene locus RP011 in contig AJ235269_GR.
KEGG	rpr:RP011.
PATRIC	17900752. VBIRicPro72556_0011.
Phylogenomic databases
HOGENOM	HBG557545.
ProtClustDB	CLSK870702.
Enzyme and pathway databases
BioCyc	RPRO272947:RP011-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR024036. tRNA-dHydroUridine_Synthase_C. IPR004652. tRNA_dU_NifR3. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1200.80. G3DSA:1.10.1200.80. 1 hit.
PANTHER	PTHR11082. Du_synth. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
TIGRFAMs	TIGR00737. NifR3_yhdG. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUS_RICPR

Accession

Primary (citable) accession number: Q9ZED2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program