Reviewed, UniProtKB/Swiss-Prot Q9ZEA8 (GCP_RICPR)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	RP037

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000096972
Sites
	Metal binding	112	1	Zinc Potential
	Metal binding	116	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZEA8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: AB974E50F138591D	FASTA	387	42,726
	10 20 30 40 50 60 MKKILGIESS CDDTAISIIT ERRKILSNII ISQNTEHAVF GGVVPEIAAR SHLSNLDQAL 70 80 90 100 110 120 KNVLKKSNTE LTEISAIAAT SGPGLIGGVI VGSMFARSLS SALKKPFIAI NHLEGHALTA 130 140 150 160 170 180 RLTDNISYPY LLLLASGGHC QFVAVLGLGK YKILGTTIDD AVGETFDKVA KMLNLSFPGG 190 200 210 220 230 240 PEIEKRAKLG NPHKYKFPKP IINSGNCNMS FSGLKTAVRT LIMNLKEVND SVINDIAASF 250 260 270 280 290 300 QFTIGAILSS KMQDAIRLYK QILNDYYEDI NHPTKLNLKS FRKDEFNWKP LECITRPKYR 310 320 330 340 350 360 IHIQNSYRSN LLNDTIVIAG GVAANKYLQE ILSDCTRPYG YRLIAPPMHL CTDNAAMIAY 370 380 AGLERYNNKL FSPLDFCPKA KWSLEDI

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14508.1.
PIR	E71711.
RefSeq	NP_220431.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	883568.
GenomeReviews	Gene locus RP037 in contig AJ235269_GR.
KEGG	rpr:RP037.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZEA8.
OMA	RNCNFSF
Enzyme and pathway databases
BioCyc	RPRO272947:RP037-MON.
BRENDA	3.4.24.57. 141977.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017860. Peptidase_M22_CS. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_RICPR

Accession

Primary (citable) accession number: Q9ZEA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)