Reviewed, UniProtKB/Swiss-Prot Q9ZEA3 (TILS_RICPR)

Names and origin

Protein names

Recommended name:
    tRNA(Ile)-lysidine synthase
    EC=6.3.4.-
Alternative name(s):
    tRNA(Ile)-lysidine synthetase
    tRNA(Ile)-2-lysyl-cytidine synthase

Gene names

Name:	tilS
Ordered Locus Names:	RP042

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	tRNA(Ile)-lysidine synthase HAMAP MF_01161		PRO_0000181757
Regions
	Nucleotide binding	27 – 32	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZEA3-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A6788F9E487D3D18	FASTA	430	50,474
	10 20 30 40 50 60 MLYEKFEYNI NNLIGNFGLS KIAIAVSGGS DSISLLYLAN IWARKNNIEL FVISVDHNLR 70 80 90 100 110 120 PQSKQENYYI HTISSNLNRK YYNLSFDHQN NFSNLQERAR EGRYDLMTNL CLELDVLVLL 130 140 150 160 170 180 TAHHEDDYVE NFCLRLERNS GIFGLSSSNI HWYNNIQIIR PLYNIPKSEL VEYLVNHKIK 190 200 210 220 230 240 WFEDESNLSD KYRRNIIRQK LFKEENYIKA EISLQQLKTN KLIEDELKPA LISAIAEAVK 250 260 270 280 290 300 IFEYGFTFLD LVKFDKFLNE VKAQIINFLL IMISGQSRSA RFYSIAHILK LISQDINFKN 310 320 330 340 350 360 TVHGCVIKRI QNELLIYREF GKKLPKSKIL LDKSVIWDNR FCITKNQKTP DCVITYLSLA 370 380 390 400 410 420 DYKAIKKQLD LKHLKNLSCK NHNAILFTLP IIKILEKVIA IPHISYYDND VWNFEVSFAP 430 NFVSRFTHFC

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14513.1.
PIR	B71712.
RefSeq	NP_220436.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883580.
GenomeReviews	Gene locus RP042 in contig AJ235269_GR.
KEGG	rpr:RP042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZEA3.
OMA	NNIQIIR
Enzyme and pathway databases
BioCyc	RPRO272947:RP042-MON.
Family and domain databases
HAMAP	MF_01161. [Tree]
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012795. Lysidine-tRNA-synth_N. IPR011063. PP_loop. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TILS_RICPR

Accession

Primary (citable) accession number: Q9ZEA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)