Q9ZEA2 (FTSH_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent zinc metalloprotease FtsH
EC=3.4.24.-

Gene names

Name:	ftsH
Ordered Locus Names:	RP043

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins By similarity. HAMAP MF_01458
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01458
Subunit structure	Homohexamer Potential.
Subcellular location	Cell inner membrane; Multi-pass membrane protein; Cytoplasmic side By similarity HAMAP MF_01458.
Sequence similarities	In the central section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein catabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	ATP-dependent zinc metalloprotease FtsH HAMAP MF_01458		PRO_0000084646
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 103	76	Periplasmic Potential
	Transmembrane	104 – 124	21	Helical; Potential
	Topological domain	125 – 637	513	Cytoplasmic Potential
	Nucleotide binding	195 – 202	8	ATP Potential
Sites
	Active site	418	1	By similarity
	Metal binding	417	1	Zinc; catalytic By similarity
	Metal binding	421	1	Zinc; catalytic By similarity
	Metal binding	495	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZEA2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5600F726DF8B44AA	FASTA	637	70,066
	10 20 30 40 50 60 MNNQGRSILA WAALFIFVIL LFNVFQSDGL LGVRNNITFS DFLTRVDEKT INSVKIQGRV 70 80 90 100 110 120 IEGTANDGST FSTYSPDYPD LVNRLTSNDV NIEVVPLETR MNTFLGFLIS WFPMLLLIGV 130 140 150 160 170 180 WVFFMRQMHG GGKAMGFGKS KARLLSDKGP KITFKDVAGI DEAKEELTEI VDFLRDPSKF 190 200 210 220 230 240 QKLGGKIPKG CLLIGPPGTG KTLLAKAIAG EANVPFFSIS GSDFVEMFVG VGASRVRDMF 250 260 270 280 290 300 EQGKRNAPCI IFIDEIDAVG RHRGIGMGGG NDEREQTLNQ MLVEMDGFEA NEGVVIIAAT 310 320 330 340 350 360 NRPDVLDRAL LRPGRFDRQI AVANPDINGR EQILKVHLKK IKYNSTVLAR IIARGTPGFS 370 380 390 400 410 420 GAELANLVNE AALIAARLGK KEVDMHDMEE AKDKVLMGVV RRSIAMSEKE KRLTAYHEGG 430 440 450 460 470 480 HALVGLYCPA ASPIHKATII PRGNALGMVQ RLPETDEYSQ NREQMESSIA VYMAGRVAEE 490 500 510 520 530 540 IIFGRNKVTS GASSDIKGAT NIARAMVTKA GLSDLIGPIF HGSNSDDMYG RQSSNEISEA 550 560 570 580 590 600 TAELIDAEVK RIITQGYEFA KDILTKHIDQ LHTLANALIE YETLSGQQIK NLLSGRALDS 610 620 630 EEENKFPFND SHTIKIDKEN LHEKVKSTKD KKENIIS

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14514.1.
PIR	C71712.
RefSeq	NP_220437.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZEA2.
SMR	Q9ZEA2. Positions 144-399.
ModBase	Search...
Protein family/group databases
MEROPS	M41.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883570.
GenomeReviews	Gene locus RP043 in contig AJ235269_GR.
KEGG	rpr:RP043.
PATRIC	17900820. VBIRicPro72556_0044.
Phylogenomic databases
HOGENOM	HBG724153.
OMA	YISDETK.
ProtClustDB	CLSK870717.
Enzyme and pathway databases
BioCyc	RPRO272947:RP043-MONOMER.
Family and domain databases
HAMAP	MF_01458. FtsH. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. Pept_M41_FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
KO	K03798.
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTSH_RICPR

Accession

Primary (citable) accession number: Q9ZEA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program