Q9ZEA1 (DHSB_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinate dehydrogenase iron-sulfur subunit
EC=1.3.99.1

Gene names

Name:	sdhB
Ordered Locus Names:	RP044

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Succinate dehydrogenase iron-sulfur subunit		PRO_0000158691
Regions
	Domain	28 – 119	92	2Fe-2S ferredoxin-type
	Domain	161 – 191	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	80	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	85	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	100	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	171	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	174	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	177	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	181	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	234	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	238	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	186	1	Ubiquinone; shared with sdhD subunit By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZEA1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: AFAB638FFF0F44A1	FASTA	261	29,572
	10 20 30 40 50 60 MVELRLPSNS VVKKGREHKA QQKMLKPRKV KVYRYDPDLD ENPTIDSFEI DLSKTGPMVL 70 80 90 100 110 120 DALIKIKNEI DSTLTFRRSC REGICGSCAM NIDGTNTLAC IKPIEDISGD IKIYPLPHMK 130 140 150 160 170 180 VVKDLVPDMS HFYAQYESIE PWLKNDSPAP SNSERLQSIK DREKLDGLYE CILCACCSTS 190 200 210 220 230 240 CPSYWWNGDK YLGPAILLQA YRWIADSRDD NTGARLEALE DPFKLYRCHT IMNCTKTCPK 250 260 GLNPAKAIGR VKNLIAERHG V

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14515.1.
PIR	D71712.
RefSeq	NP_220438.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZEA1.
SMR	Q9ZEA1. Positions 28-256.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883577.
GenomeReviews	Gene locus RP044 in contig AJ235269_GR.
KEGG	rpr:RP044.
PATRIC	17900822. VBIRicPro72556_0045.
Phylogenomic databases
HOGENOM	HBG616382.
OMA	IQVTQAI.
ProtClustDB	PRK05950.
Enzyme and pathway databases
BioCyc	RPRO272947:RP044-MONOMER.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. Beta-grasp_ferredoxin-type. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KO	K00240.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR00384. DhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_RICPR

Accession

Primary (citable) accession number: Q9ZEA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program