Reviewed, UniProtKB/Swiss-Prot Q9ZE90 (MNMG_RICPR)

Names and origin

Protein names

Recommended name:
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Alternative name(s):
    Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	RP056

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG HAMAP MF_00129		PRO_0000117166
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity
	Nucleotide binding	269 – 283	15	NAD Potential
Sites
	Binding site	122	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	177	1	FAD By similarity
	Binding site	366	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE90-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 7379A85B4E6ACADF	FASTA	621	69,195
	10 20 30 40 50 60 MLKYGVIIIG GGHAGVEAAA ASARLGVHTL LITLKPENLG EMSCNPAIGG IAKGTLVKEI 70 80 90 100 110 120 DALDGLMGFV IDKSGIHYKM LNETRGPAVW GPRAQADRKL YKKVMYQILT NYRNLDILYA 130 140 150 160 170 180 KVEDIQIKSS KVEAVILSNG SKIFCQKVVL TTGTFLSGFI HIGSIKMPAG RIYEEPSYGL 190 200 210 220 230 240 SNTLKRLGFK IARLKTGTPP RIDGRTIDYS KTALQQGDQI PRPFSELTDV IDVPQINCFI 250 260 270 280 290 300 TKTTSETHDI IRENLDKSAM YSGQIEGIGP RYCPSIEDKI VKFSTKLEHR IFLEPEGLED 310 320 330 340 350 360 YTIYPNGIST SLPEEVQYKL IKTIPGLENA QVLRPGYAIE YDYVDPREIN VTLETKKITG 370 380 390 400 410 420 LYFAGQINGT TGYEEAAGQG IIAGINAALS VKDQAPFILT RATSYIGVMI DDLTTFGTVE 430 440 450 460 470 480 PYRMFTSRSE YRLSLRADNA DLRLTELGIK IGVITEKRKK FFTKKCKNIE KTKLLLNNLS 490 500 510 520 530 540 LTTSKLAKMG IQVAQDGKYK TILDLFKIPS FNVEQAIKIF PILKKQNNNI LQLLYIEAKY 550 560 570 580 590 600 ASYLTRQYAD INLFQSEEIQ LIPKNIDYFK IPSISLEIQE KLSYHKPATI GVARRISGIT 610 620 PASITAIIIY LKTKYSDESS K

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14527.1.
PIR	H71713.
RefSeq	NP_220450.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883597.
GenomeReviews	Gene locus RP056 in contig AJ235269_GR.
KEGG	rpr:RP056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284774.
OMA	IQFRVLN.
Enzyme and pathway databases
BioCyc	RPRO272947:RP056-MON.
Family and domain databases
HAMAP	MF_00129. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_RICPR

Accession

Primary (citable) accession number: Q9ZE90

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	December 15, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)