Q9ZE79 (PARC_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 4 subunit A
EC=5.99.1.3

Alternative name(s):
Topoisomerase IV subunit A

Gene names

Name:	parC
Ordered Locus Names:	RP067

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity. HAMAP-Rule MF_00936
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_00936
Subunit structure	Heterotetramer composed of ParC and ParE By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00936.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	DNA topoisomerase 4 subunit A HAMAP-Rule MF_00936		PRO_0000145406
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
	Site	40	1	Interaction with DNA By similarity
	Site	76	1	Interaction with DNA By similarity
	Site	78	1	Interaction with DNA By similarity
	Site	119	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE79 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 166B9B88C76E52A8	FASTA	738	83,348
	10 20 30 40 50 60 MKEAKIENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG 70 80 90 100 110 120 YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY 130 140 150 160 170 180 TESRMTEICM LLMEDIDKDT VDFRSTYDDS DLEPVIMPAS FPNLLANGSE GIAVGMATNI 190 200 210 220 230 240 PPHNLHELCD ALLYLIDNPQ AGINDIMNFI KGPDFPTGGI IIDKAEVINA AYTTGRGSFR 250 260 270 280 290 300 VRSRWEKEEL SYGTYQIVVT EIPYQIQKSK LIEQIAILLK DKKIPLISSI RDESTDIIRV 310 320 330 340 350 360 VIEPRDRSCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNILEIL QEFLVHRKNI 370 380 390 400 410 420 IIRRSTYLLN KIKQRLEILK VLRIVYLNLD EIIEIIREED EPKTIIMERF KISAIQVEVI 430 440 450 460 470 480 LNTRLRSLQK LEEHAIIDEH SNLQKQQAIL EKILKNHKEL WQIVKKEIKA VQTKFGLNTI 490 500 510 520 530 540 IGARRTSFEE VDLTNQVVDI TAFITKEPIT IICSKMGWVR SLKGHNTDLS TIKYKEGDTE 550 560 570 580 590 600 KFIIEAYTTD KILIISSKGR FFTLLADNIS KGKGTGGVSI KLLVDIGNND ITNILVYKPN 610 620 630 640 650 660 QLLLLASSIG KGFLVNSNEV IAQTKTGKQI MNIPEGYSCI ACLPVNGDSI ACIGESRRLL 670 680 690 700 710 720 VFNIDEIPEM KKGQGVVLQR FKNAKLLDIK IFNKQDGLSW NDGTKIQLEK NIVAFLGKRG 730 GFGTFPPIGF PKNNRFSP

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14538.1.
PIR	C71715.
RefSeq	NP_220461.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE79.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14538; CAA14538; CAA14538.
GeneID	883632.
KEGG	rpr:RP067.
PATRIC	17900868. VBIRicPro72556_0068.
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076277.
KO	K02621.
OMA	KYKDGGL.
ProtClustDB	PRK05561.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_00936. ParC_type1.
InterPro	IPR024946. Arg_repress_C-like. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. IPR005742. TopoIV_A_Gneg. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 3 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01062. parC_Gneg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PARC_RICPR

Accession

Primary (citable) accession number: Q9ZE79

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program