Reviewed, UniProtKB/Swiss-Prot Q9ZE74 (QUEF_RICPR)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	RP072

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163054

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE74-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 410FFFE8E2147B8C	FASTA	273	31,481
	10 20 30 40 50 60 MPLSTSLLGK KNTYKDSYDA TLLFKIPRIN NRNVLGIDSN HLPFYGVDIW NTYEISCLNK 70 80 90 100 110 120 NGKPLVGIGT FYIPADSENI VESKSFKLYL NSFNNFIIES IEELERIILQ DLSNVTYAKV 130 140 150 160 170 180 TGRIFPINTK IEFGIPSGKN IDNLDIVCNN YGPPDNSLIE YEDVLVEEEI YSNLFKSNCL 190 200 210 220 230 240 VTGQPDWGTI VIKYKGKKLK YDSFLRYLIS FRNFNEFAEQ CAERIFIDIK NSINLDFLSI 250 260 270 YIVYTRRGGI DICPYRSTDK SYTLPNDKRL IRQ

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14543.1.
PIR	H71715.
RefSeq	NP_220466.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883641.
GenomeReviews	Gene locus RP072 in contig AJ235269_GR.
KEGG	rpr:RP072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZE74.
OMA	INPFRSN
Enzyme and pathway databases
BioCyc	RPRO272947:RP072-MON.
BRENDA	1.7.1.13. 141977.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICPR

Accession

Primary (citable) accession number: Q9ZE74

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)