Q9ZE70 (SAM_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine uptake transporter

Gene names

Name:	sam
Ordered Locus Names:	RP076

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transports S-adenosylmethionine. Ref.1
Enzyme regulation	Transport is inhibited by S-adenosylethionine and to a lesser extent by S-adenosylhomocysteine. Unlike eukaryotic transporters is not inhibited by sinfungin. Also inhibited by 2.4-dinitrophenol, suggesting transport is an energy-dependent process. Ref.1
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the drug/metabolite transporter (DMT) superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family. [View classification] Contains 2 DUF6 domains.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	S-adenosylmethionine uptake transporter		PRO_0000280997
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	74 – 91	18	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	260 – 280	21	Helical; Potential
	Domain	22 – 141	120	DUF6 1
	Domain	160 – 280	121	DUF6 2

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE70 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3DE6527258466AB1	FASTA	294	33,092
	10 20 30 40 50 60 MNDALKTYLN GICWFLLSLV TSSINDVMSK YLGTRLHSFE VAFFRFFFSS IVLLPFVVYY 70 80 90 100 110 120 GKNALKTSRP FVHVLRGLLL FFGMTSWTYG LTIAPVTTAT VVSFAIPLFT LILAVFILNE 130 140 150 160 170 180 NIIWQRWVVT VVGFIGLVVM LKPHTKDFNP EILYLILAAI SFAMLDIINK KFVVKESMLS 190 200 210 220 230 240 MLFYSAIVTA MVSLPVAMQY WITPSSFELA LLFVLGSSGS FILFFLLKAF SIVDATATAP 250 260 270 280 290 YRYLELVISA IAAYFIFNEF PDKSTVHGAV IIIPATLFII YSEKKSMSSK HESQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S-adenosylmethionine transport in Rickettsia prowazekii." Tucker A.M., Winkler H.H., Driskell L.O., Wood D.O. J. Bacteriol. 185:3031-3035(2003) [PubMed: 12730161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14547.1.
PIR	D71716.
RefSeq	NP_220470.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE70.
ModBase	Search...
Protein family/group databases
TCDB	2.A.7.3.7. drug/metabolite transporter (DMT) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883647.
GenomeReviews	Gene locus RP076 in contig AJ235269_GR.
KEGG	rpr:RP076.
PATRIC	17900886. VBIRicPro72556_0077.
Phylogenomic databases
HOGENOM	HBG559298.
OMA	QRWVVTV.
ProtClustDB	CLSK870734.
Enzyme and pathway databases
BioCyc	RPRO272947:RP076-MONOMER.
Family and domain databases
InterPro	IPR000620. DMT. [Graphical view]
KO	K15270.
Pfam	PF00892. EamA. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAM_RICPR

Accession

Primary (citable) accession number: Q9ZE70

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program