Q9ZE62 (SYC_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	RP085

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159469
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	275	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE62 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1BEC03E8C6AA5F81	FASTA	457	53,303
	10 20 30 40 50 60 MKLHLYNTLT RTKEVFNPQD PANIKMYVCG PTVYDNPHIG NSRSVVVYDL LYRIIIKIFG 70 80 90 100 110 120 KKAVKYVRNI TDVDDKIIDR AELLGITIND LTDKVTREFH INMEYLGCML PTIEPKATEH 130 140 150 160 170 180 IDVMIEIIER LIAKDHAYIA DNHVYFDVLS APNYTELSNR NLEAMFEGVR IENSKTKKNP 190 200 210 220 230 240 QDFVLWKPAK KNEPENINFT SPWGLGRPGW HIECSAMSYK YLGQNFDIHG GGADLIFPHH 250 260 270 280 290 300 TNEIAQSRCA FPSSTYAKYW IHNGFLTVNG SKMSKSLGNF ITVRDLMDKQ IQGEVVRLFL 310 320 330 340 350 360 LSSHYRRPLD YNDKAIDDAK KTLDYWYRAI EHINVQKVDL PSNFMRSLFD DMNTPLAIKI 370 380 390 400 410 420 INDYAKCVFI SKTEAERKFN ASAIITCANF IGLMRKTQYE WFNRDVDEFY INELINKRLK 430 440 450 AKKQKNWLLA DKIRNQLLEK KIILEDKSNY ITIWRKE

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14555.1.
PIR	D71717.
RefSeq	NP_220478.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE62.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883669.
GenomeReviews	Gene locus RP085 in contig AJ235269_GR.
KEGG	rpr:RP085.
PATRIC	17900904. VBIRicPro72556_0085.
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	RPRO272947:RP085-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_RICPR

Accession

Primary (citable) accession number: Q9ZE62

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program