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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A (By similarity).By similarity

Catalytic activityi

Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathway:iLPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021Proton acceptorBy similarity
Sitei172 – 1721Transition state stabilizerBy similarity
Sitei247 – 2471Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.12)
Short name:
Kdo transferase
Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:kdtA
Ordered Locus Names:RP089
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221Helical; Signal-anchorSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4614613-deoxy-D-manno-octulosonic acid transferasePRO_0000080290Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272947.RP089.

Structurei

3D structure databases

ProteinModelPortaliQ9ZE58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 8842RPE1 insertAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni306 – 3072CMP-Kdo bindingBy similarity
Regioni347 – 3493CMP-Kdo bindingBy similarity
Regioni372 – 3754CMP-Kdo bindingBy similarity

Sequence similaritiesi

Contains 1 RPE1 insert domain.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1519.
HOGENOMiHOG000010628.
KOiK02527.
OMAiQHCYLPY.
OrthoDBiEOG6H7FNN.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZE58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLYYTLSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSALD
60 70 80 90 100
FIQMSVNKEG FTDHKTTSYV DMHRNASLMY KLSLERSYAH SLVWIHAASV
110 120 130 140 150
GEVMTALTLI HNISKLAPNV RFLITSWTNA SAKILSTKLP KIATHQFLPI
160 170 180 190 200
DNVIFTRKFL RNWQPDLGIF IESELWPCTI NEGAKYCKLL LINARISNKS
210 220 230 240 250
FKAWLKRKRF FQLIIKNFSK IIVQSERDLQ KFNALGISDA MNLGNIKFAN
260 270 280 290 300
EKLLVNQEKL SKLILHLDNR RVLVFASTHP EDEEVILPII NNLKEQFIDC
310 320 330 340 350
YIILIPRHPE RIKSIINNCK LHHLSATAKS QNDLPVLNND LYIVDRFGEM
360 370 380 390 400
GLFFSVATIS FIGGSFKQGG HNILEAAYFS NCIIFGPDMS KNTDIAKGIL
410 420 430 440 450
QNNAAIQIKN GKDLLNTLTS LLNANNALKL KTYRENALKF VENNQKKILD
460
EYLQIIKQFL P
Length:461
Mass (Da):52,976
Last modified:May 1, 1999 - v1
Checksum:iBE027A7C2D34AC28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14559.1.
PIRiH71717.
RefSeqiNP_220482.1. NC_000963.1.
WP_004599735.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14559; CAA14559; CAA14559.
GeneIDi883675.
KEGGirpr:RP089.
PATRICi17900912. VBIRicPro72556_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14559.1.
PIRiH71717.
RefSeqiNP_220482.1. NC_000963.1.
WP_004599735.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZE58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP089.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA14559; CAA14559; CAA14559.
GeneIDi883675.
KEGGirpr:RP089.
PATRICi17900912. VBIRicPro72556_0089.

Phylogenomic databases

eggNOGiCOG1519.
HOGENOMiHOG000010628.
KOiK02527.
OMAiQHCYLPY.
OrthoDBiEOG6H7FNN.

Enzyme and pathway databases

UniPathwayiUPA00958.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.
  2. Cited for: DOMAIN RPE1.

Entry informationi

Entry nameiKDTA_RICPR
AccessioniPrimary (citable) accession number: Q9ZE58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.