Q9ZE58 (KDTA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonic acid transferase
Short name=Kdo transferase
EC=2.4.99.12

Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RP089

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.
Catalytic activity	Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	3-deoxy-D-manno-octulosonic acid transferase		PRO_0000080290
Regions
	Transmembrane	2 – 22	21	Helical; Signal-anchor; Potential
	Domain	47 – 88	42	RPE1 insert
	Region	306 – 307	2	CMP-Kdo binding By similarity
	Region	347 – 349	3	CMP-Kdo binding By similarity
	Region	372 – 375	4	CMP-Kdo binding By similarity
Sites
	Active site	102	1	Proton acceptor By similarity
	Site	172	1	Transition state stabilizer By similarity
	Site	247	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE58 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BE027A7C2D34AC28	FASTA	461	52,976
	10 20 30 40 50 60 MMLLYYTLSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSALD FIQMSVNKEG 70 80 90 100 110 120 FTDHKTTSYV DMHRNASLMY KLSLERSYAH SLVWIHAASV GEVMTALTLI HNISKLAPNV 130 140 150 160 170 180 RFLITSWTNA SAKILSTKLP KIATHQFLPI DNVIFTRKFL RNWQPDLGIF IESELWPCTI 190 200 210 220 230 240 NEGAKYCKLL LINARISNKS FKAWLKRKRF FQLIIKNFSK IIVQSERDLQ KFNALGISDA 250 260 270 280 290 300 MNLGNIKFAN EKLLVNQEKL SKLILHLDNR RVLVFASTHP EDEEVILPII NNLKEQFIDC 310 320 330 340 350 360 YIILIPRHPE RIKSIINNCK LHHLSATAKS QNDLPVLNND LYIVDRFGEM GLFFSVATIS 370 380 390 400 410 420 FIGGSFKQGG HNILEAAYFS NCIIFGPDMS KNTDIAKGIL QNNAAIQIKN GKDLLNTLTS 430 440 450 460 LLNANNALKL KTYRENALKF VENNQKKILD EYLQIIKQFL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Selfish DNA in protein-coding genes of Rickettsia." Ogata H., Audic S., Barbe V., Artiguenave F., Fournier P.-E., Raoult D., Claverie J.-M. Science 290:347-350(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN RPE1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14559.1.
PIR	H71717.
RefSeq	NP_220482.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE58.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP089.
Protein family/group databases
CAZy	GT30. Glycosyltransferase Family 30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14559; CAA14559; CAA14559.
GeneID	883675.
KEGG	rpr:RP089.
PATRIC	17900912. VBIRicPro72556_0089.
Phylogenomic databases
eggNOG	COG1519.
HOGENOM	HOG000010628.
KO	K02527.
OMA	KHGGHNP.
ProtClustDB	PRK05749.
Enzyme and pathway databases
UniPathway	UPA00958.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. [Graphical view]
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDTA_RICPR

Accession

Primary (citable) accession number: Q9ZE58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program