Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9ZE58 (KDTA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-deoxy-D-manno-octulosonic acid transferase

Short name=Kdo transferase
EC=2.4.99.12
Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene names
Name:waaA
Synonyms:kdtA
Ordered Locus Names:RP089
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.

Catalytic activity

Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Subcellular location

Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily.

Contains 1 RPE1 insert domain.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipopolysaccharide core region biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4614613-deoxy-D-manno-octulosonic acid transferase
PRO_0000080290

Regions

Transmembrane2 – 2221Helical; Signal-anchor; Potential
Domain47 – 8842RPE1 insert
Region306 – 3072CMP-Kdo binding By similarity
Region347 – 3493CMP-Kdo binding By similarity
Region372 – 3754CMP-Kdo binding By similarity

Sites

Active site1021Proton acceptor By similarity
Site1721Transition state stabilizer By similarity
Site2471Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZE58 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: BE027A7C2D34AC28

FASTA46152,976
        10         20         30         40         50         60 
MMLLYYTLSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSALD FIQMSVNKEG 

        70         80         90        100        110        120 
FTDHKTTSYV DMHRNASLMY KLSLERSYAH SLVWIHAASV GEVMTALTLI HNISKLAPNV 

       130        140        150        160        170        180 
RFLITSWTNA SAKILSTKLP KIATHQFLPI DNVIFTRKFL RNWQPDLGIF IESELWPCTI 

       190        200        210        220        230        240 
NEGAKYCKLL LINARISNKS FKAWLKRKRF FQLIIKNFSK IIVQSERDLQ KFNALGISDA 

       250        260        270        280        290        300 
MNLGNIKFAN EKLLVNQEKL SKLILHLDNR RVLVFASTHP EDEEVILPII NNLKEQFIDC 

       310        320        330        340        350        360 
YIILIPRHPE RIKSIINNCK LHHLSATAKS QNDLPVLNND LYIVDRFGEM GLFFSVATIS 

       370        380        390        400        410        420 
FIGGSFKQGG HNILEAAYFS NCIIFGPDMS KNTDIAKGIL QNNAAIQIKN GKDLLNTLTS 

       430        440        450        460 
LLNANNALKL KTYRENALKF VENNQKKILD EYLQIIKQFL P 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.
[2]"Selfish DNA in protein-coding genes of Rickettsia."
Ogata H., Audic S., Barbe V., Artiguenave F., Fournier P.-E., Raoult D., Claverie J.-M.
Science 290:347-350(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN RPE1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235270 Genomic DNA. Translation: CAA14559.1.
PIRH71717.
RefSeqNP_220482.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZE58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP089.

Protein family/group databases

CAZyGT30. Glycosyltransferase Family 30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA14559; CAA14559; CAA14559.
GeneID883675.
KEGGrpr:RP089.
PATRIC17900912. VBIRicPro72556_0089.

Phylogenomic databases

eggNOGCOG1519.
HOGENOMHOG000010628.
KOK02527.
OMALPYDLPC.
OrthoDBEOG6H7FNN.

Enzyme and pathway databases

UniPathwayUPA00958.

Family and domain databases

InterProIPR007507. Glycos_transf_N.
[Graphical view]
PfamPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKDTA_RICPR
AccessionPrimary (citable) accession number: Q9ZE58
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways