Reviewed, UniProtKB/Swiss-Prot Q9ZE58 (KDTA_RICPR)

Names and origin

Protein names

Recommended name:
    3-deoxy-D-manno-octulosonic-acid transferase
    EC=2.4.-.-
Alternative name(s):
    KDO transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RP089

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA) By similarity.
Pathway	Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 1/4. Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 2/4. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 1 family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sugar binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	3-deoxy-D-manno-octulosonic-acid transferase		PRO_0000080290
Regions
	Transmembrane	2 – 22	21	Signal-anchor Potential
	Domain	47 – 88	42	RPE1 insert

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE58-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BE027A7C2D34AC28	FASTA	461	52,976
	10 20 30 40 50 60 MMLLYYTLSF ILLPVYFIII FIRLLIGKED IRRIQERFAI GKQRQNSALD FIQMSVNKEG 70 80 90 100 110 120 FTDHKTTSYV DMHRNASLMY KLSLERSYAH SLVWIHAASV GEVMTALTLI HNISKLAPNV 130 140 150 160 170 180 RFLITSWTNA SAKILSTKLP KIATHQFLPI DNVIFTRKFL RNWQPDLGIF IESELWPCTI 190 200 210 220 230 240 NEGAKYCKLL LINARISNKS FKAWLKRKRF FQLIIKNFSK IIVQSERDLQ KFNALGISDA 250 260 270 280 290 300 MNLGNIKFAN EKLLVNQEKL SKLILHLDNR RVLVFASTHP EDEEVILPII NNLKEQFIDC 310 320 330 340 350 360 YIILIPRHPE RIKSIINNCK LHHLSATAKS QNDLPVLNND LYIVDRFGEM GLFFSVATIS 370 380 390 400 410 420 FIGGSFKQGG HNILEAAYFS NCIIFGPDMS KNTDIAKGIL QNNAAIQIKN GKDLLNTLTS 430 440 450 460 LLNANNALKL KTYRENALKF VENNQKKILD EYLQIIKQFL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Selfish DNA in protein-coding genes of Rickettsia." Ogata H., Audic S., Barbe V., Artiguenave F., Fournier P.-E., Raoult D., Claverie J.-M. Science 290:347-350(2000) [PubMed: 11030655] [Abstract] Cited for: DOMAIN RPE1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235270 Genomic DNA. Translation: CAA14559.1.
PIR	H71717.
RefSeq	NP_220482.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT30. Glycosyltransferase Family 30.
Genome annotation databases
GeneID	883675.
GenomeReviews	Gene locus RP089 in contig AJ235269_GR.
KEGG	rpr:RP089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZE58.
OMA	VQHCYLP
Enzyme and pathway databases
BioCyc	RPRO272947:RP089-MON.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. [Graphical view]
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDTA_RICPR

Accession

Primary (citable) accession number: Q9ZE58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)