Q9ZE20 (RPOC_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	RP141

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	1372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1372	1372	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067786
Natural variations
	Natural variant	33	1	S → G in strain: Breinl.

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE20 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 7241DF46696F465C	FASTA	1,372	153,382
	10 20 30 40 50 60 MSVVNFYGQL SNTQQFDQIR INIASPDQVR SWSFGEVTKP ETINYRTFKP EKDGLFCARI 70 80 90 100 110 120 FGPVKDYECL CGKYKRMKNR GITCEKCGVE VTVSRVRRER MGHIELAAPV AHIWFLKSLP 130 140 150 160 170 180 SRISTLLDMT MRDVEKILYF ENYVVVDPGL SILQKGELLT EEELQKAKDK YGEDAFTASI 190 200 210 220 230 240 GAEVIQQMLK ELDFSKLKQE LYDELHITSS EVKKKKLVKR LKLVEDFLES ENKPEWMIMD 250 260 270 280 290 300 VLPVIPPEIR PLVMLDGGRF ATSDLNELYR RVINRNNRLK KLIESKAPDI IVRNEKRMLQ 310 320 330 340 350 360 EAVDALFDNG RRGRAAKNAN KRPFKSLSDM LKGKQGRFRQ NLLGKRVDYS GRSVIVVGPE 370 380 390 400 410 420 LKLHQCGLPK KMALELFKPF IYSKLELYGI ATTIKAAKRM VEAEKPEVWD VLEEVIREHP 430 440 450 460 470 480 VLLNRAPTLH RLGIQAFEPL LIEGKAIQLH PLVCAAFNAD FDGDQMAVHI PLSIEAQLEA 490 500 510 520 530 540 RVFMMSTNNI LSPANGRPII VPDKDIVLGL YYLTIAFDNE VGEGMMFSDL AEMEHALYNK 550 560 570 580 590 600 FITIHTKIKY RRDQLNAEGK MVPVIIDTTY GRLMVGELLP SNPNIEFKFI NKQLTKKDIS 610 620 630 640 650 660 LVIDLVYRHC GQKATVIFAD QLMKLGFKYA CSSGISFGMD DMVVPESKST HINETQLEIK 670 680 690 700 710 720 EFEQQYSNGL ITYGEKYNKV VDAWSRCTDR VANDMMKEIA TPPVNDYPNH QKINAIYMMA 730 740 750 760 770 780 ISGARGSFQQ IKQLGGMRGL MTKSNGQIIQ TPIISNFKEG LTEFECFNSA NGMRKGQIDT 790 800 810 820 830 840 ALKTASSGYL TRKLVDVAQD CIITEKDCGT DKGIEVKSVI EGGEIIVPLA EKILGRTAAI 850 860 870 880 890 900 DIFHPVTNDL ILNKGELINE SKLEQIESAG LDRIMIKSVL TCESSTGICS ICYGRDLATG 910 920 930 940 950 960 TLVSEGEAIG VIAAQSIGEP GTQLTMRTFH IGGAATKGAE VSSVEASYDA KVKIISRNVV 970 980 990 1000 1010 1020 INSEERKIVM SRNCELLLLD NNGNEKARHK IPYGARLLVD DGDMVIKTQK LAEWDPYTIP 1030 1040 1050 1060 1070 1080 IITEKSGKVL FKDMVEGISI RDVTDEATGI PSKVIIESKQ YSRGAELRPR IQLLDSKGEV 1090 1100 1110 1120 1130 1140 ITLSNGLEAR YYLPVGAVLS VEDGIQISVG DIIARIPKES TTTKDITGGL PRVAELVEAR 1150 1160 1170 1180 1190 1200 RPKDHAVIAE VDGRVEFGKD YKSKRRIIIH PIDGTMSIEY MVPKGKHVVV NEGDFVKKGD 1210 1220 1230 1240 1250 1260 LLIDGNPVLQ DILKVMGVEV LANYIVKEVQ AVYRLQGVKI DDKHIEVIIR QMLQKVEVTD 1270 1280 1290 1300 1310 1320 SGGTTLLVGE KIDRHEFDEI NAKAMKNGLK PAEAQLILQG ITKASLQTRS FISAASFQET 1330 1340 1350 1360 1370 TRVLTEAAIA GKVDKLRGLK ENVIVGRLVP AGTGYFMDKM RKAAVKLDEE NV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Characterization of mutations in the rpoB gene in naturally rifampin-resistant Rickettsia species." Drancourt M., Raoult D. Antimicrob. Agents Chemother. 43:2400-2403(1999) [PubMed: 10508014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33. Strain: ATCC VR-142 / Breinl.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14609.1. AF076437 Genomic DNA. Translation: AAF22440.1.
PIR	B71724.
RefSeq	NP_220532.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE20.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883787.
GenomeReviews	Gene locus RP141 in contig AJ235269_GR.
KEGG	rpr:RP141.
PATRIC	17901024. VBIRicPro72556_0143.
Phylogenomic databases
HOGENOM	HBG621785.
OMA	DINDKHI.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	RPRO272947:RP141-MONOMER.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_RICPR

Accession

Primary (citable) accession number: Q9ZE20
Secondary accession number(s): Q9RH36

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program