Q9ZE10 (GATA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-

Gene names

Name:	gatA
Ordered Locus Names:	RP152

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120		PRO_0000105197
Sites
	Active site	78	1	Charge relay system By similarity
	Active site	158	1	Charge relay system By similarity
	Active site	182	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZE10 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 885F71DA7A49DF0D	FASTA	493	53,259
	10 20 30 40 50 60 MTELNKLTVA ESVKGLKNKD FTSKELVNAY IKQIEKYKNL NAYVTETFDL ALKQAEAADQ 70 80 90 100 110 120 NYGQNKARTL EGIPFAVKDL FCTKGIRTTA CSNILKNFIP HYESSVTQNI FDKGGVILGK 130 140 150 160 170 180 TNMDEFAMGS ANITSCFGNV ISPWKANDDN SDLVPGGSSG GSAAAVSAFM ASAALGSDTG 190 200 210 220 230 240 GSVRQPASFT GLVGFKPTYG RCSRYGMVSF ASSLDQAGIF TRSVLDSSIM LEAMMGFDEK 250 260 270 280 290 300 DSTSINAQVP ELQSAIGSSM KNMKIGVPLS LGEGGIIEHD IMKMWHDTIE LLKNAGAEIV 310 320 330 340 350 360 DITLPHAKYG VAVYYVIAPA EAASNLSRYD GVRYGLRVEC ENMTLDEMYE MTRSAGFGEE 370 380 390 400 410 420 VKRRIMLGTY VLSSHCMDAY YFKAQKVRRL VANDFNNAFA KVNAILLPTA PSAAFKIGKK 430 440 450 460 470 480 QNDPTIMYLN DLFTIPASLA GLPCASVPAG LSARGLPLGM QIIGKQLDEY NVLKVASTIE 490 TGVKHIKFEP AGF

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14620.1.
PIR	E71725.
RefSeq	NP_220543.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZE10.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883817.
GenomeReviews	Gene locus RP152 in contig AJ235269_GR.
KEGG	rpr:RP152.
PATRIC	17901052. VBIRicPro72556_0157.
Phylogenomic databases
HOGENOM	HBG481888.
OMA	FDEETLF.
ProtClustDB	PRK00012.
Enzyme and pathway databases
BioCyc	RPRO272947:RP152-MONOMER.
Family and domain databases
HAMAP	MF_00120. GatA. [Tree]
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K02433.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. GatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATA_RICPR

Accession

Primary (citable) accession number: Q9ZE10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program