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Protein

Ribosomal RNA large subunit methyltransferase E

Gene

rlmE

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei80 – 801S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Binding sitei143 – 1431S-adenosyl-L-methionineUniRule annotation
Active sitei183 – 1831Proton acceptorUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase EUniRule annotation (EC:2.1.1.166UniRule annotation)
Alternative name(s):
23S rRNA Um2552 methyltransferaseUniRule annotation
rRNA (uridine-2'-O-)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmEUniRule annotation
Synonyms:ftsJUniRule annotation, rrmJUniRule annotation
Ordered Locus Names:RP163
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Ribosomal RNA large subunit methyltransferase EPRO_0000155534Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272947.RP163.

Structurei

3D structure databases

ProteinModelPortaliQ9ZE00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0293.
HOGENOMiHOG000162367.
KOiK02427.
OMAiLRTMHLC.
OrthoDBiEOG657JC0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZE00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNLSGYRN KIVRVKTSKK RPISSSNWLR RQLNDPYVAK ARLEGFRSRA
60 70 80 90 100
AYKLLEIHYK FRLFTPNMKI VDLGAAPGGW SQVASKLIKA SDNNLNNKII
110 120 130 140 150
SIDLLEIEPI VGVEFLQKDF FEKDTEELII HNLEGKADIV MSDMASNTIG
160 170 180 190 200
HKATDHMRTL LLCEQAFEFA LKVLKPSCHF IAKIFRGGAE TALLNKVKRE
210 220
FRTVKHFKPV SSRSESTEIY LVALNKK
Length:227
Mass (Da):25,881
Last modified:May 1, 1999 - v1
Checksum:i3A9CCA205D925118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14630.1.
PIRiG71726.
RefSeqiNP_220553.1. NC_000963.1.
WP_004595881.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14630; CAA14630; CAA14630.
GeneIDi883842.
KEGGirpr:RP163.
PATRICi17901074. VBIRicPro72556_0167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14630.1.
PIRiG71726.
RefSeqiNP_220553.1. NC_000963.1.
WP_004595881.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZE00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA14630; CAA14630; CAA14630.
GeneIDi883842.
KEGGirpr:RP163.
PATRICi17901074. VBIRicPro72556_0167.

Phylogenomic databases

eggNOGiCOG0293.
HOGENOMiHOG000162367.
KOiK02427.
OMAiLRTMHLC.
OrthoDBiEOG657JC0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiRLME_RICPR
AccessioniPrimary (citable) accession number: Q9ZE00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.