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Q9ZE00 (RLME_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase E

EC=2.1.1.166
Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase
Gene names
Name:rlmE
Synonyms:ftsJ, rrmJ
Ordered Locus Names:RP163
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547

Catalytic activity

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01547.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA (uridine-2'-O-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547
PRO_0000155534

Sites

Active site1831Proton acceptor By similarity
Binding site781S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site801S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZE00 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3A9CCA205D925118

FASTA22725,881
        10         20         30         40         50         60 
MTNNLSGYRN KIVRVKTSKK RPISSSNWLR RQLNDPYVAK ARLEGFRSRA AYKLLEIHYK 

        70         80         90        100        110        120 
FRLFTPNMKI VDLGAAPGGW SQVASKLIKA SDNNLNNKII SIDLLEIEPI VGVEFLQKDF 

       130        140        150        160        170        180 
FEKDTEELII HNLEGKADIV MSDMASNTIG HKATDHMRTL LLCEQAFEFA LKVLKPSCHF 

       190        200        210        220 
IAKIFRGGAE TALLNKVKRE FRTVKHFKPV SSRSESTEIY LVALNKK 

« Hide

References

[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235270 Genomic DNA. Translation: CAA14630.1.
PIRG71726.
RefSeqNP_220553.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZE00.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP163.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA14630; CAA14630; CAA14630.
GeneID883842.
KEGGrpr:RP163.
PATRIC17901074. VBIRicPro72556_0167.

Phylogenomic databases

eggNOGCOG0293.
HOGENOMHOG000162367.
KOK02427.
OMAQYVVSQI.
OrthoDBEOG657JC0.
ProtClustDBCLSK870770.

Family and domain databases

HAMAPMF_01547. RNA_methyltr_E.
InterProIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
[Graphical view]
PANTHERPTHR10920. PTHR10920. 1 hit.
PfamPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFPIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLME_RICPR
AccessionPrimary (citable) accession number: Q9ZE00
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names