Q9ZDY5 (QUED_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase
Short name=CPH4 synthase
EC=4.1.2.50

Alternative name(s):
Queuosine biosynthesis protein QueD

Gene names

Name:	queD
Ordered Locus Names:	RP178

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit By similarity.
Sequence similarities	Belongs to the PTPS family. QueD subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	6-carboxy-5,6,7,8-tetrahydropterin synthase		PRO_0000057928
Sites
	Active site	23	1	Proton acceptor By similarity
	Active site	68	1	Charge relay system By similarity
	Active site	130	1	Charge relay system By similarity
	Metal binding	14	1	Zinc By similarity
	Metal binding	27	1	Zinc By similarity
	Metal binding	29	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDY5 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 014B19A34593B968	FASTA	138	16,227
	10 20 30 40 50 60 MIKCTRRINF DAGHRIIGHK NKCQFLHGHH YVLEITIAAN KTDTLGMVID FGLIKNLAKK 70 80 90 100 110 120 WIDANFDHNL ILHQDDKEMG QQIENYTRQK IYYMRNNPTA ENIATHLKNE IFPKLFVSQN 130 FFVTSLKLYE TQNCFVEV

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14645.1.
PIR	F71728.
RefSeq	NP_220568.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDY5.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP178.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14645; CAA14645; CAA14645.
GeneID	883870.
KEGG	rpr:RP178.
PATRIC	17901106. VBIRicPro72556_0183.
Phylogenomic databases
eggNOG	COG0720.
HOGENOM	HOG000225068.
KO	K01737.
OMA	KWIDANF.
ProtClustDB	CLSK870781.
Enzyme and pathway databases
UniPathway	UPA00391.
Family and domain databases
InterPro	IPR007115. 6-PTP_synth/QueD. [Graphical view]
PANTHER	PTHR12589. PTHR12589. 1 hit.
Pfam	PF01242. PTPS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

QUED_RICPR

Accession

Primary (citable) accession number: Q9ZDY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program