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Protein

UvrABC system protein B

Gene

uvrB

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 458ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:RP203
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662UvrABC system protein BPRO_0000138421Add
BLAST

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP203.

Structurei

3D structure databases

ProteinModelPortaliQ9ZDW2.
SMRiQ9ZDW2. Positions 2-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 414390Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini430 – 592163Helicase C-terminalUniRule annotationAdd
BLAST
Domaini622 – 65736UVRUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 11424Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0556.
HOGENOMiHOG000073580.
KOiK03702.
OMAiFETKMRQ.
OrthoDBiEOG6B360R.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZDW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNFSIISGY KPAGDQPKAI DEIITGLNSK KRSQMLLGIT GSGKTFTMAN
60 70 80 90 100
IIERTNRPTL IMAHNKTLAA QIYLEMKSIF PKNAVEYFVS YYDYYQPESY
110 120 130 140 150
IVRTDTFIEK DSSINEQIDL MRHSATRSLL ERRDVIVISS VSCIYGLGAP
160 170 180 190 200
DLYYQMTVHL EPGQNYPRDK LLNDLINLQY KRNDIGFERG CFRVKGDNMD
210 220 230 240 250
IFPSHYSDKA WRLSFFGDEL EYIHEFDPLT GEKLTQLDKA MIFGNSHFVM
260 270 280 290 300
PRERINHAIS SIEVELQKRL EFLKSQDKLI EAKRLNQRTL YDLEMLTETG
310 320 330 340 350
SCKGIENYSR FFTGRNAGQP PPTLFEYLPE DALLFIDESH VSVPQIRAMY
360 370 380 390 400
NSDRARKEVL VEHGFRLPSA LDNRPLKFEE WEKFRPQTVF VSATPGQFEL
410 420 430 440 450
EETGGTVVEL IIRPTGLLDP ECIIKPATNQ VEDLISEIQT TINTGLRILV
460 470 480 490 500
TTLTKKMAED LTSYLQDLQY KTYYLHSNIH TLERIEILRN LRQGTIDILV
510 520 530 540 550
GINLLREGID IPECGLVAIL DADKEGFLRS ETSLIQTIGR AARNSRGKVI
560 570 580 590 600
LYADKMTKSI DKAVSETLRR RQIQQEYNKK HGIIPKTINR AIHALESLEE
610 620 630 640 650
IHDNKLDKKQ ANALLNNPAK LKAHMDKLKK EMFKAASNLE FEQAAKLRNQ
660
LKALEEAALK LS
Length:662
Mass (Da):75,859
Last modified:May 1, 1999 - v1
Checksum:i3C58A49A5602421F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14668.1.
PIRiE71731.
RefSeqiNP_220591.1. NC_000963.1.
WP_004598584.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14668; CAA14668; CAA14668.
GeneIDi883916.
KEGGirpr:RP203.
PATRICi17901164. VBIRicPro72556_0212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA. Translation: CAA14668.1.
PIRiE71731.
RefSeqiNP_220591.1. NC_000963.1.
WP_004598584.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZDW2.
SMRiQ9ZDW2. Positions 2-593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP203.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA14668; CAA14668; CAA14668.
GeneIDi883916.
KEGGirpr:RP203.
PATRICi17901164. VBIRicPro72556_0212.

Phylogenomic databases

eggNOGiCOG0556.
HOGENOMiHOG000073580.
KOiK03702.
OMAiFETKMRQ.
OrthoDBiEOG6B360R.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiUVRB_RICPR
AccessioniPrimary (citable) accession number: Q9ZDW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 27, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.