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Q9ZDW2 (UVRB_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B

Short name=Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene names
Name:uvrB
Ordered Locus Names:RP203
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length662 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 UVR domain.

Ontologies

Keywords
   Biological processDNA damage
DNA excision
DNA repair
SOS response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionExcision nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSOS response

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide-excision repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

excinuclease ABC activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 662662UvrABC system protein B HAMAP-Rule MF_00204
PRO_0000138421

Regions

Domain25 – 414390Helicase ATP-binding
Domain430 – 592163Helicase C-terminal
Domain622 – 65736UVR
Nucleotide binding38 – 458ATP Potential
Motif91 – 11424Beta-hairpin HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
Q9ZDW2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3C58A49A5602421F

FASTA66275,859
        10         20         30         40         50         60 
MNNFSIISGY KPAGDQPKAI DEIITGLNSK KRSQMLLGIT GSGKTFTMAN IIERTNRPTL 

        70         80         90        100        110        120 
IMAHNKTLAA QIYLEMKSIF PKNAVEYFVS YYDYYQPESY IVRTDTFIEK DSSINEQIDL 

       130        140        150        160        170        180 
MRHSATRSLL ERRDVIVISS VSCIYGLGAP DLYYQMTVHL EPGQNYPRDK LLNDLINLQY 

       190        200        210        220        230        240 
KRNDIGFERG CFRVKGDNMD IFPSHYSDKA WRLSFFGDEL EYIHEFDPLT GEKLTQLDKA 

       250        260        270        280        290        300 
MIFGNSHFVM PRERINHAIS SIEVELQKRL EFLKSQDKLI EAKRLNQRTL YDLEMLTETG 

       310        320        330        340        350        360 
SCKGIENYSR FFTGRNAGQP PPTLFEYLPE DALLFIDESH VSVPQIRAMY NSDRARKEVL 

       370        380        390        400        410        420 
VEHGFRLPSA LDNRPLKFEE WEKFRPQTVF VSATPGQFEL EETGGTVVEL IIRPTGLLDP 

       430        440        450        460        470        480 
ECIIKPATNQ VEDLISEIQT TINTGLRILV TTLTKKMAED LTSYLQDLQY KTYYLHSNIH 

       490        500        510        520        530        540 
TLERIEILRN LRQGTIDILV GINLLREGID IPECGLVAIL DADKEGFLRS ETSLIQTIGR 

       550        560        570        580        590        600 
AARNSRGKVI LYADKMTKSI DKAVSETLRR RQIQQEYNKK HGIIPKTINR AIHALESLEE 

       610        620        630        640        650        660 
IHDNKLDKKQ ANALLNNPAK LKAHMDKLKK EMFKAASNLE FEQAAKLRNQ LKALEEAALK 


LS 

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References

[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235270 Genomic DNA. Translation: CAA14668.1.
PIRE71731.
RefSeqNP_220591.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZDW2.
SMRQ9ZDW2. Positions 2-593.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP203.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA14668; CAA14668; CAA14668.
GeneID883916.
KEGGrpr:RP203.
PATRIC17901164. VBIRicPro72556_0212.

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMAYEQEHSD.
OrthoDBEOG6B360R.
ProtClustDBPRK05298.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUVRB_RICPR
AccessionPrimary (citable) accession number: Q9ZDW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names