Q9ZDS8 (MURC_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	RP247

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP-Rule MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00046
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	UDP-N-acetylmuramate--L-alanine ligase HAMAP-Rule MF_00046		PRO_0000182143
Regions
	Nucleotide binding	120 – 126	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDS8 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2E18464088FAD2D6	FASTA	495	54,613
	10 20 30 40 50 60 MLLLELKKTN QTLGTIHFIG IGGVGMSGIA EILHNLGYKV QGSDLVENYN TKRLESYGIK 70 80 90 100 110 120 IFLGQAKQNI KNVSYVVISS AINQNNPEIK EALERKIPII RRAEMLAELM RLKCSVAVSG 130 140 150 160 170 180 SHGKTTTTSL IACLFEAAGL CPTVINGGII NNKSTNAYLG SSNYLIAEAD ESDATFIHIP 190 200 210 220 230 240 STIAIITNID PEHLDYYQDF EILIGAFRSF ITNLPFYGFA VCCIDHKIVR KLVDDITERK 250 260 270 280 290 300 IITYGIDSED AHIIAFNINT DIASSTFDVK ISLPNVLGTT IIEKITIPTP GRHNILNSLA 310 320 330 340 350 360 AIAVGIELDF GIKAIKNGFN NFKGVKRRFT KVAEYNKAVI IDDYAHHPEE IKATLATAKN 370 380 390 400 410 420 IANQQNGKVI AIFQPHRYSR IKYLFDDFML CFADADILYI TNIYAAGETP IEGITGQSLV 430 440 450 460 470 480 DKMTQNKHHD KANFLAELDD VVSIIIDNAA SGDMIIMMGA GNISSFANEL DRRLLSHEIL 490 ENTDCDTKSN DKVMQ

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14709.1.
PIR	C71679.
RefSeq	NP_220632.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDS8.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP247.
Proteomic databases
PRIDE	Q9ZDS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14709; CAA14709; CAA14709.
GeneID	884023.
KEGG	rpr:RP247.
PATRIC	17901254. VBIRicPro72556_0254.
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HOG000256031.
KO	K01924.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.40.50.720. 1 hit. 3.90.190.20. 1 hit.
HAMAP	MF_00046. MurC.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_RICPR

Accession

Primary (citable) accession number: Q9ZDS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program