Reviewed, UniProtKB/Swiss-Prot Q9ZDQ5 (UCRI_RICPR)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Ordered Locus Names:	RP270

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127766
Regions
	Transmembrane	18 – 38	21	Potential
	Domain	88 – 175	88	Rieske
Sites
	Metal binding	120	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	122	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	139	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	142	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	125 ↔ 141		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDQ5-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: D6DA19D703A90B39	FASTA	177	19,350
	10 20 30 40 50 60 MSDTEDNKNK QTTRRDFIVL TASSVAAVGA ACAFWPIIDS FNPSADVLAL SSIEVDLSNI 70 80 90 100 110 120 AIGQTVTVKW QGKPIFITNR THDEIAAARA VKMSELIDPE RDEVRVKAGH DNWLVTIGIC 130 140 150 160 170 THLGCVPLSH KGEYNGWFCP CHGSQYDSSG RVRKGPAPLN LAVPPYIFIS DKKIRIG

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235271 Genomic DNA. Translation: CAA14732.1.
PIR	B71682.
RefSeq	NP_220655.1.
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
ModBase	Search...
Genome annotation databases
GeneID	883187.
GenomeReviews	Gene locus RP270 in contig AJ235269_GR.
KEGG	rpr:RP270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZDQ5.
OMA	Q9ZDQ5. TTIDVAN.
Enzyme and pathway databases
BioCyc	RPRO272947:RP270-MON.
BRENDA	1.10.2.2. 141977.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_RICPR

Accession

Primary (citable) accession number: Q9ZDQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)