Reviewed, UniProtKB/Swiss-Prot Q9ZDL2 (DHAS_RICPR)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	RP316

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Aspartate-semialdehyde dehydrogenase		PRO_0000141388
Sites
	Active site	132	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDL2-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 61E4CE8DDAD7A20E	FASTA	338	37,668
	10 20 30 40 50 60 MTKKYNIAVI GATGNVGRET LNILAERHFP INKIYAIASD NSLGREVRFG EKILHINSIK 70 80 90 100 110 120 IFNFHDIEIA FFCAGSNVSK EFIPKATSCN CIVIDKTSLF RADNQVPLIV PEVNLSTLKE 130 140 150 160 170 180 FNTKNIIANP NCIVIPLVVV LKPLDNEIKI KRVVISTYQS VSGAGKAGMD ELYNQTKSKY 190 200 210 220 230 240 VFRENNPKKF PKQIAFNLFP YIGDLNKDGY TSEETKIAFE LNKIMGNHFK TSVTSVRVPV 250 260 270 280 290 300 FIGHAISVNI EFSDKIYAKD VEEILQDADG IVTISNNNGL AYISPIEVVG EDAVYVSRIR 310 320 330 NDLSKDNTIN LWITCDNLRK GAALNSVQIA EALINNYL

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235271 Genomic DNA. Translation: CAA14776.1.
PIR	F71687.
RefSeq	NP_220699.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883494.
GenomeReviews	Gene locus RP316 in contig AJ235269_GR.
KEGG	rpr:RP316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZDL2.
OMA	Q9ZDL2. KPLDNEI.
Enzyme and pathway databases
BioCyc	RPRO272947:RP316-MON.
BRENDA	1.2.1.11. 141977.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_RICPR

Accession

Primary (citable) accession number: Q9ZDL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)