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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.By similarity

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.182)
Gene namesi
Name:lpxB
Ordered Locus Names:RP321
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Lipid-A-disaccharide synthasePRO_0000190182Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272947.RP321.

Structurei

3D structure databases

ProteinModelPortaliQ9ZDK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiYYIIAGE.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZDK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIYFIAGE MSGDFIGGHV IQNLKSNEGL EFTGIGGKYM EEAGNFKSLF
60 70 80 90 100
TITAINLIGF IEIIPHLLKI KKLIDKTVEH IINSKADLLI TIDSPGFTYR
110 120 130 140 150
VAKRVRKLLP NLKMIHIVAP SVWAYKADRA VNYAKIYDCL FALLPFEPPY
160 170 180 190 200
FTKVGLDCRY IGHPIMEQEF YRDKIALRKE LKIDENERIL CVTLGTRKGE
210 220 230 240 250
ILRHLPIFID AIQEISKDYK NLTIIFPLAH PDHEAIIKPF LDNIQFNYLF
260 270 280 290 300
LSNERLKAYA VSDLALAKSG TNTLEISASG TPMVVAYKVN IISFIIIMLL
310 320 330 340 350
IKIKYVSLIN IIAGSEIIPE FIQFNCKANL ISNKLKELLS NSQKRYNQVV
360 370 380
KSKKILQKLG FESNRSPSYI AAKIIKQEFL
Length:380
Mass (Da):43,249
Last modified:May 1, 1999 - v1
Checksum:iE8718E4C5E6ECDD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235271 Genomic DNA. Translation: CAA14781.1.
PIRiC71688.
RefSeqiNP_220704.1. NC_000963.1.
WP_004597431.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14781; CAA14781; CAA14781.
GeneIDi883381.
KEGGirpr:RP321.
PATRICi17901409. VBIRicPro72556_0330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235271 Genomic DNA. Translation: CAA14781.1.
PIRiC71688.
RefSeqiNP_220704.1. NC_000963.1.
WP_004597431.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZDK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP321.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA14781; CAA14781; CAA14781.
GeneIDi883381.
KEGGirpr:RP321.
PATRICi17901409. VBIRicPro72556_0330.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018004.
KOiK00748.
OMAiYYIIAGE.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiLPXB_RICPR
AccessioniPrimary (citable) accession number: Q9ZDK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.