Reviewed, UniProtKB/Swiss-Prot Q9ZDK3 (SYE1_RICPR)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	RP325

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000119640
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDK3-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: DC3467D259DFB5F9	FASTA	447	52,348
	10 20 30 40 50 60 MTKVITRFAP SPTGMLHVGN IRVALLNWLY AKKHNGKFIL RFDDTDLERS KQKYKNDIER 70 80 90 100 110 120 DLKFLNINWD QTFNQLSRVS RYHEIKNLLI NKKRLYACYE TKEELELKRK LQLSKGLPPI 130 140 150 160 170 180 YDRASLNLTE KQIQKYIEQG RKPHYRFFLS YEPISWFDMI KGEIKYDGKT LSDPIVIRAD 190 200 210 220 230 240 GSMTYMLCSV IDDIDYDITH IIRGEDHVSN TAIQIQMFEA LNKIPPVFAH LSLIINKEEK 250 260 270 280 290 300 ISKRVGGFEI AYLKKEVGLE AMTIASFFSL LGSSLHIFPY KSIEKLVAQF EISSFSKSPT 310 320 330 340 350 360 IYQQYDLERL NHKLLISLDF NEVKERLKEI DADYIDENFW LSVRPNLQKL SDIKDWWDIC 370 380 390 400 410 420 YQTPKIKNLN LDKEYLKQAS KLLPLKITKD SWSIWTKEIT NITGRKGKEL FLPLRLALTG 430 440 RESGPEIAGI LPLIDREEII RRLISIA

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235271 Genomic DNA. Translation: CAA14785.1.
PIR	G71688.
RefSeq	NP_220708.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYL based on UniProtKB P00962.
ModBase	Search...
Genome annotation databases
GeneID	883400.
GenomeReviews	Gene locus RP325 in contig AJ235269_GR.
KEGG	rpr:RP325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZDK3.
OMA	LRLDDTD.
Enzyme and pathway databases
BioCyc	RPRO272947:RP325-MON.
BRENDA	6.1.1.17. 141977.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RICPR

Accession

Primary (citable) accession number: Q9ZDK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)