Q9ZDK2 (TOP1_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	RP326

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	DNA topoisomerase 1		PRO_0000145163
Regions
	Domain	1 – 114	114	Toprim
	Zinc finger	600 – 627	28	C4-type
Sites
	Active site	304	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDK2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C0F738A3691B7CCE	FASTA	776	88,722
	10 20 30 40 50 60 MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDKNFLM EYDISDKAGK 70 80 90 100 110 120 YVDAIVKEAR KAEVVYLATD PDREGESISW HVAEVIKEKN KVESDDFFKR VAFNEITKKA 130 140 150 160 170 180 IMNAVANPRK LDTNLVNAQQ ARRALDYLVG FTLSPLLWRK LPGCKSAGRV QSVALRLICD 190 200 210 220 230 240 REDEIERFKS EEYWDISLKM QNSNNDLFTA KLTHVNDQKL KKFSIINEKE AKDLTQKLKL 250 260 270 280 290 300 QKFYVEKIEK KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG 310 320 330 340 350 360 LITYMRTDGV TLSNDAIADI RKLIDKNYGN QYLPIKPRIY QSKVKNAQEA HEAIRPTNIT 370 380 390 400 410 420 YTPDSLKQKL EKDYYKLYEL IWHRTIACQM ENVIMDLVIA NLASENKEYL AKANGSIIAF 430 440 450 460 470 480 DGFYKVYRES LDDEDEEDNK MLPPLKEQEH IKTKEVIPNK HFTEPPPRYS EASLVKKLEE 490 500 510 520 530 540 LGIGRPSTYA SILSVLQDRK YVALEKKRFI PEELGRLVTV FLVGFFKKYV EYDFTAGLEN 550 560 570 580 590 600 ELDEIAAGKL EWKTALNNFW RGFNHNIESV NEQKITEIIN YLQKALDYHL FGEDKESKVC 610 620 630 640 650 660 PSCKTGQLSL KLGKFGAFLA CSNYPECTFK KSIVSGNDNN EDEGDPSTIL NDNKILGTDQ 670 680 690 700 710 720 DGVEIYLKKG PYGPYIQLGE QCGKVKPKRT PVPANLKQSE ITLEIALKLL NLPLKIGIHK 730 740 750 760 770 DSGEEIIIGY SKFGPYIKYM CKFISVPKKY DFLNLSLNDA MKLIQNNKAK LEKKYG

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14786.1.
PIR	H71688.
RefSeq	NP_220709.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDK2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883482.
GenomeReviews	Gene locus RP326 in contig AJ235269_GR.
KEGG	rpr:RP326.
PATRIC	17901421. VBIRicPro72556_0336.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Enzyme and pathway databases
BioCyc	RPRO272947:RP326-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_RICPR

Accession

Primary (citable) accession number: Q9ZDK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program