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Q9ZDK2 (TOP1_RICPR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:RP326
OrganismRickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]
Taxonomic identifier272947 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length776 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 776776DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145163

Regions

Domain1 – 111111Toprim
Zinc finger600 – 62728C4-type HAMAP-Rule MF_00952
Region166 – 1716Interaction with DNA By similarity

Sites

Active site3041O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding71Magnesium 1; catalytic By similarity
Metal binding801Magnesium 1; catalytic By similarity
Metal binding801Magnesium 2 By similarity
Metal binding821Magnesium 2 By similarity
Site311Interaction with DNA By similarity
Site1421Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site1461Interaction with DNA By similarity
Site1581Interaction with DNA By similarity
Site3061Interaction with DNA By similarity
Site4991Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ZDK2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: C0F738A3691B7CCE

FASTA77688,722
        10         20         30         40         50         60 
MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDKNFLM EYDISDKAGK 

        70         80         90        100        110        120 
YVDAIVKEAR KAEVVYLATD PDREGESISW HVAEVIKEKN KVESDDFFKR VAFNEITKKA 

       130        140        150        160        170        180 
IMNAVANPRK LDTNLVNAQQ ARRALDYLVG FTLSPLLWRK LPGCKSAGRV QSVALRLICD 

       190        200        210        220        230        240 
REDEIERFKS EEYWDISLKM QNSNNDLFTA KLTHVNDQKL KKFSIINEKE AKDLTQKLKL 

       250        260        270        280        290        300 
QKFYVEKIEK KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG 

       310        320        330        340        350        360 
LITYMRTDGV TLSNDAIADI RKLIDKNYGN QYLPIKPRIY QSKVKNAQEA HEAIRPTNIT 

       370        380        390        400        410        420 
YTPDSLKQKL EKDYYKLYEL IWHRTIACQM ENVIMDLVIA NLASENKEYL AKANGSIIAF 

       430        440        450        460        470        480 
DGFYKVYRES LDDEDEEDNK MLPPLKEQEH IKTKEVIPNK HFTEPPPRYS EASLVKKLEE 

       490        500        510        520        530        540 
LGIGRPSTYA SILSVLQDRK YVALEKKRFI PEELGRLVTV FLVGFFKKYV EYDFTAGLEN 

       550        560        570        580        590        600 
ELDEIAAGKL EWKTALNNFW RGFNHNIESV NEQKITEIIN YLQKALDYHL FGEDKESKVC 

       610        620        630        640        650        660 
PSCKTGQLSL KLGKFGAFLA CSNYPECTFK KSIVSGNDNN EDEGDPSTIL NDNKILGTDQ 

       670        680        690        700        710        720 
DGVEIYLKKG PYGPYIQLGE QCGKVKPKRT PVPANLKQSE ITLEIALKLL NLPLKIGIHK 

       730        740        750        760        770 
DSGEEIIIGY SKFGPYIKYM CKFISVPKKY DFLNLSLNDA MKLIQNNKAK LEKKYG 

« Hide

References

[1]"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ235271 Genomic DNA. Translation: CAA14786.1.
PIRH71688.
RefSeqNP_220709.1. NC_000963.1.

3D structure databases

ProteinModelPortalQ9ZDK2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272947.RP326.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAA14786; CAA14786; CAA14786.
GeneID883482.
KEGGrpr:RP326.
PATRIC17901421. VBIRicPro72556_0336.

Phylogenomic databases

eggNOGCOG1754.
HOGENOMHOG000004020.
KOK03168.
OMAVNDQKLE.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK06599.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_RICPR
AccessionPrimary (citable) accession number: Q9ZDK2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names