Reviewed, UniProtKB/Swiss-Prot Q9ZDH2 (NUOB_RICPR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B
    NDH-1 subunit B

Gene names

Name:	nuoB
Ordered Locus Names:	RP356

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	NADH-quinone oxidoreductase subunit B		PRO_0000118779
Sites
	Metal binding	47	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	48	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	112	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	142	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDH2-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A4745BE28523C2E2	FASTA	174	19,659
	10 20 30 40 50 60 MEHDFYQEDE LLNSGLTNRG FLLSKVDEVI SWARANSLWP MTFGLACCAV EMMQAAASRY 70 80 90 100 110 120 DMDRFGMLFR PSPRQSDLMI VAGTLTNKMA PALRKVYDQM TEPKWVLSMG SCANGGGYYH 130 140 150 160 170 FSYSVVRGCD RIVPVDVYVP GCPPTAEALI YGLMQLQKKI KRTTGFKYDS RQTH

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235271 Genomic DNA. Translation: CAA14816.1.
PIR	F71692.
RefSeq	NP_220740.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	883398.
GenomeReviews	Gene locus RP356 in contig AJ235269_GR.
KEGG	rpr:RP356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZDH2.
Enzyme and pathway databases
BioCyc	RPRO272947:RP356-MON.
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

NUOB_RICPR

Accession

Primary (citable) accession number: Q9ZDH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	November 4, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)