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Protein

Probable NADP-dependent malic enzyme

Gene

RP373

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971Proton acceptorBy similarity
Metal bindingi139 – 1391Divalent metal cationBy similarity
Metal bindingi140 – 1401Divalent metal cationBy similarity
Binding sitei165 – 1651NADBy similarity
Binding sitei290 – 2901NADBy similarity

GO - Molecular functioni

  1. malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  2. malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB-EC
  3. manganese ion binding Source: InterPro
  4. NAD binding Source: InterPro
  5. oxaloacetate decarboxylase activity Source: UniProtKB-EC
  6. transferase activity, transferring acyl groups Source: InterPro

GO - Biological processi

  1. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Gene namesi
Ordered Locus Names:RP373
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000002480: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 767767Probable NADP-dependent malic enzymePRO_0000160247Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272947.RP373.

Structurei

3D structure databases

ProteinModelPortaliQ9ZDF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 430430Malic enzymeAdd
BLAST
Regioni431 – 767337Phosphate acetyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the malic enzymes family.Curated
In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0281.
HOGENOMiHOG000132448.
KOiK00029.
OMAiGYLYRDC.
OrthoDBiEOG6QCD9W.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR012188. ME_PTA.
IPR016040. NAD(P)-bd_dom.
IPR002505. PTA_PTB.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
PF01515. PTA_PTB. 1 hit.
[Graphical view]
PIRSFiPIRSF036684. ME_PTA. 1 hit.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ZDF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEMNKINYT EALEYHEKDK PGKIAITTTK SLVTQQDLSL AYSPGVAAPC
60 70 80 90 100
LEISKNLEAV YKYTSRSNLV AVISNGTAVL GLGNLGAAAS KPVMEGKAVL
110 120 130 140 150
FKKFADIDAI DLEVNTEDPI EFINAVKYLG YSFGGINLED IKAPECFLIE
160 170 180 190 200
EKLKSLMDIP VFHDDQHGTA IITAAGLINA AYLTNRTLKD LKIVINGAGA
210 220 230 240 250
AAIACIDLLI ALGVDKSKII LCDTKGVIYK GRTSGMNKWK ERYASDTKIR
260 270 280 290 300
TLTESLNNAD VFIGLSVKGA VTKDMISKMA HKPIIFAMAN PDPEITPEDI
310 320 330 340 350
KFVRDDAIIA TGRSDYNNQV NNVMGFPYIF RGALDVRAST INTEMKIAAA
360 370 380 390 400
RAIADLARRP VPEEVYKAYS GRKMVFGNEY IIPVPFDPRL ITVVATAVAV
410 420 430 440 450
AAIESGVARV KDFSIDKYKQ QLGSRLNPTA NYMNFLAEKI HNVPLKRIVF
460 470 480 490 500
AEGEEEEVIS AALMMRDEKY GNPIIIGRVE RIEVTLKKIG KDISLAGIQI
510 520 530 540 550
MNAALSDRLE QYTDYLYKRL QRKGYLYRDC AKLVKTDKNI FAACMVACGD
560 570 580 590 600
GDALLTGVTK SYIDSLEDII KVISPKQNRR ILGYSIMIAK DHNIIIADNC
610 620 630 640 650
ITEYPNSLEL AQIATQTAEI AKNMGITPRV ALIAFSTFGN SSQEKTVRIR
660 670 680 690 700
EAVNILDNFS KDKKKLNGIK VDFEYDGEMS VKVALDHDLR KLYQFCRLSG
710 720 730 740 750
SANVLIMPGL NSAAISTELL QKFSSNSFIG PITNGFAKPV QILPTTATAN
760
EILKIATFAC VEAIKEV
Length:767
Mass (Da):84,253
Last modified:May 1, 1999 - v1
Checksum:i6782D33C85E30A5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235271 Genomic DNA. Translation: CAA14832.1.
PIRiF71694.
RefSeqiNP_220756.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA14832; CAA14832; CAA14832.
GeneIDi883450.
KEGGirpr:RP373.
PATRICi17901519. VBIRicPro72556_0384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235271 Genomic DNA. Translation: CAA14832.1.
PIRiF71694.
RefSeqiNP_220756.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZDF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA14832; CAA14832; CAA14832.
GeneIDi883450.
KEGGirpr:RP373.
PATRICi17901519. VBIRicPro72556_0384.

Phylogenomic databases

eggNOGiCOG0281.
HOGENOMiHOG000132448.
KOiK00029.
OMAiGYLYRDC.
OrthoDBiEOG6QCD9W.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR012188. ME_PTA.
IPR016040. NAD(P)-bd_dom.
IPR002505. PTA_PTB.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
PF01515. PTA_PTB. 1 hit.
[Graphical view]
PIRSFiPIRSF036684. ME_PTA. 1 hit.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Madrid E.

Entry informationi

Entry nameiMAO2_RICPR
AccessioniPrimary (citable) accession number: Q9ZDF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.