Reviewed, UniProtKB/Swiss-Prot Q9ZDF6 (MAO2_RICPR)

Names and origin

Protein names

Recommended name:
    Probable NADP-dependent malic enzyme
      Short name=NADP-ME
    EC=1.1.1.40

Gene names

Ordered Locus Names:

RP373

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Sequence similarities	In the N-terminal section; belongs to the malic enzymes family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro acyltransferase activity Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Probable NADP-dependent malic enzyme		PRO_0000160247
Regions
	Region	1 – 430	430	Malic enzyme
	Region	431 – 767	337	Phosphate acetyltransferase
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	139	1	Divalent metal cation By similarity
	Metal binding	140	1	Divalent metal cation By similarity
	Binding site	165	1	NAD By similarity
	Binding site	290	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDF6-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6782D33C85E30A5E	FASTA	767	84,253
	10 20 30 40 50 60 MDEMNKINYT EALEYHEKDK PGKIAITTTK SLVTQQDLSL AYSPGVAAPC LEISKNLEAV 70 80 90 100 110 120 YKYTSRSNLV AVISNGTAVL GLGNLGAAAS KPVMEGKAVL FKKFADIDAI DLEVNTEDPI 130 140 150 160 170 180 EFINAVKYLG YSFGGINLED IKAPECFLIE EKLKSLMDIP VFHDDQHGTA IITAAGLINA 190 200 210 220 230 240 AYLTNRTLKD LKIVINGAGA AAIACIDLLI ALGVDKSKII LCDTKGVIYK GRTSGMNKWK 250 260 270 280 290 300 ERYASDTKIR TLTESLNNAD VFIGLSVKGA VTKDMISKMA HKPIIFAMAN PDPEITPEDI 310 320 330 340 350 360 KFVRDDAIIA TGRSDYNNQV NNVMGFPYIF RGALDVRAST INTEMKIAAA RAIADLARRP 370 380 390 400 410 420 VPEEVYKAYS GRKMVFGNEY IIPVPFDPRL ITVVATAVAV AAIESGVARV KDFSIDKYKQ 430 440 450 460 470 480 QLGSRLNPTA NYMNFLAEKI HNVPLKRIVF AEGEEEEVIS AALMMRDEKY GNPIIIGRVE 490 500 510 520 530 540 RIEVTLKKIG KDISLAGIQI MNAALSDRLE QYTDYLYKRL QRKGYLYRDC AKLVKTDKNI 550 560 570 580 590 600 FAACMVACGD GDALLTGVTK SYIDSLEDII KVISPKQNRR ILGYSIMIAK DHNIIIADNC 610 620 630 640 650 660 ITEYPNSLEL AQIATQTAEI AKNMGITPRV ALIAFSTFGN SSQEKTVRIR EAVNILDNFS 670 680 690 700 710 720 KDKKKLNGIK VDFEYDGEMS VKVALDHDLR KLYQFCRLSG SANVLIMPGL NSAAISTELL 730 740 750 760 QKFSSNSFIG PITNGFAKPV QILPTTATAN EILKIATFAC VEAIKEV

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14832.1.
PIR	F71694.
RefSeq	NP_220756.1.
3D structure databases
SMR	Q9ZDF6. Positions 4-403, 436-765.
ModBase	Search...
Genome annotation databases
GeneID	883450.
KEGG	rpr:RP373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289132.
OMA	YPFCRLS.
Enzyme and pathway databases
BioCyc	RPRO272947:RP373-MONOMER.
BRENDA	1.1.1.40. 141977.
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR012188. ME_PTA. IPR016040. NAD(P)-bd_dom. IPR002505. PTA_PTB. [Graphical view]
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF036684. ME_PTA. 1 hit.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO2_RICPR

Accession

Primary (citable) accession number: Q9ZDF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)