Q9ZDF3 (MDH_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	RP376

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Malate dehydrogenase HAMAP MF_00487		PRO_0000113469
Regions
	Nucleotide binding	11 – 16	6	NAD By similarity
	Nucleotide binding	120 – 122	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	35	1	NAD By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	97	1	NAD By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDF3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 53FB298E235ED35B	FASTA	314	34,175
	10 20 30 40 50 60 MKKNPKISLI GSGNIGGTLA HLISLKKLGD IVLFDVSEGL PQGKALDLMQ AATIEGSDIK 70 80 90 100 110 120 IKGTNDYRDI EGSDAVIITA GLPRKPGMSR DDLISVNTKI MKDVAQNIKK YAQNAFVIVI 130 140 150 160 170 180 TNPLDIMVYV MLKESGLPHN KVIGMAGVLD SSRFNLFLAK EFKVSVKNVN SIVLGGHGDT 190 200 210 220 230 240 MVPLLRYSTI SGVPIPDLIK MGLSSNKNIE KIIDRTKNGG GEIVKLLKTG SAYYAPAASA 250 260 270 280 290 300 IAMLESYLKD KRQILTCAAY LQGEYDIHDL YIGVPIIIGK EGVIKVIELQ LTEEEKILFY 310 KSVTEVKKLI DTIQ

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14835.1.
PIR	A71695.
RefSeq	NP_220759.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDF3.
SMR	Q9ZDF3. Positions 1-314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883393.
GenomeReviews	Gene locus RP376 in contig AJ235269_GR.
KEGG	rpr:RP376.
PATRIC	17901527. VBIRicPro72556_0388.
Phylogenomic databases
HOGENOM	HBG566126.
OMA	EIVVITA.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	RPRO272947:RP376-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_RICPR

Accession

Primary (citable) accession number: Q9ZDF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program