Q9ZDB5 (SYFA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	RP417

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000126752
Sites
	Metal binding	259	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZDB5 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C3B7FCA42CB1C7AD	FASTA	350	40,758
	10 20 30 40 50 60 MKNIETILKL AEEKIVLVHN LKDLQEYKVE FLGRNGIVTS ELKKLGSLIN GQKRKEFGLK 70 80 90 100 110 120 INTLKDKIHD IIKAKAQNLE AEELHLKLAA DKIDLTIPAR RYKQGSIHPI TQCMDELIQV 130 140 150 160 170 180 FSQFGFTIEN GPNIENDFYN FTALNFEYDH PARQMHDTFY LKGHENDKPL LLRTHTSTVQ 190 200 210 220 230 240 IRAMKNGKPP FRFIAPGRTY RSDSDMTHTP MFHQIEGLVI DKNINMGHLK YVIITFIRSF 250 260 270 280 290 300 FENSNIELRF RPSFFPFTEP SAEVDIRMNK NDKWLEVLGC GMVHPNVLKN IDINRSEYQG 310 320 330 340 350 FAFGLGVERF AMLKYNIKDL RRFFEGDIRW LKHYNFESFD IPNLAGGLTK

References

[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14874.1.
PIR	H71699.
RefSeq	NP_220798.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDB5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883326.
GenomeReviews	Gene locus RP417 in contig AJ235269_GR.
KEGG	rpr:RP417.
PATRIC	17901615. VBIRicPro72556_0430.
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	RPRO272947:RP417-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_RICPR

Accession

Primary (citable) accession number: Q9ZDB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program