Phenylalanine--tRNA ligase beta chain - Rickettsia prowazekii (strain Madrid E)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	RP418

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 815

815

Phenylalanine--tRNA ligase beta chain HAMAP MF_00283

PRO_0000126940

Regions

Domain

39 – 148

110

tRNA-binding

Domain

421 – 496

76

B5

Domain

721 – 814

94

FDX-ACB

Sites

Metal binding

474

1

Magnesium By similarity

Metal binding

480

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

483

1

Magnesium By similarity

Metal binding

484

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ZDB4 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: F508E95333FCAE43
Show »

FASTA

815

91,585

        10         20         30         40         50         60 
MKFTLSWLKQ FLEISASVTE IAEALTDIGL EVEEVIDKSK ELQKFEVAYI RNIKPHPSAD 

        70         80         90        100        110        120 
KLKLCDVETK NGILQIVCGA SNVRADIKVV LANIGIEIPK GNLKIKESVI RGQKSYGMLC 

       130        140        150        160        170        180 
SEEELLLSSN SDGIIELPKD AVVGDNFTKY YGLDDPIFVI NVTPNRGDVL GVYGIARDLS 

       190        200        210        220        230        240 
AKGLGTLKEL ELSEIKSTFF SKIKLNVHDK EACPLFTFRE IRNLKNKPSP NWLQQLLKNV 

       250        260        270        280        290        300 
GIKTISSLVD VTNYISHSFG QPIHAYDADK IYGGISVDCY IRSDKVISCK NHEMATAVLQ 

       310        320        330        340        350        360 
FSNDSANFYA INGKGYLLTE NDLAIKDESG IQGLAGIIGG AKSSCNDSTT NVILEAACFN 

       370        380        390        400        410        420 
AKMVAASGRR LKIDTDARYR NERNIDRNFT EKALNIATNL ILSICGNCEV SEVVKVGEQE 

       430        440        450        460        470        480 
PQKKPLDFSV YFLEKITGIK LSIQEIEDIL NKLGFITDVK GDIIKVIAPS WRHDINILED 

       490        500        510        520        530        540 
IAEEIVRIYG YDKIESIKLP ELYQNNNLRE YKRISSFKRI LASKGYDEVV TNSFMSSEDA 

       550        560        570        580        590        600 
KLFAELKEGL FLLNPMSIEE NYMRPTVLPN LISIVSKNLA RDVKDMAFFE VGPSFVNLNI 

       610        620        630        640        650        660 
ESTYLTAIIS GAFNNKNPHS FGRNYDIFDI KGDLEQVIEY AGLSLDKCIV IDETVLPQYY 

       670        680        690        700        710        720 
HPTRAINIRL GKNLLGHFGQ IHPKILKYYD INQEIFAFEL NITNLPLIKA KFGKRDEFTV 

       730        740        750        760        770        780 
SDYQANFRDY SFIVDQDHKV GEIISYIKNF NKKLVKSVML FDIYSGDKLP EGKKSIAIKI 

       790        800        810 
KLQADDRTLS ETDLNSFSED LVASISQKFQ GILRE

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References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria."
Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G.
Nature 396:133-140(1998) [PubMed: 9823893] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14875.1.
PIR	A71700.
RefSeq	NP_220799.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZDB4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883323.
GenomeReviews	Gene locus RP418 in contig AJ235269_GR.
KEGG	rpr:RP418.
PATRIC	17901617. VBIRicPro72556_0431.
Phylogenomic databases
HOGENOM	HBG399638.
OMA	ADKLRVC.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	RPRO272947:RP418-MONOMER.
Family and domain databases
HAMAP	MF_00283. Phe_tRNA_synth_beta1. [Tree]
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004532. Phe-tRNA-synth_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.50.40.10. B3_4. 1 hit. G3DSA:3.30.56.20. B5. 1 hit. G3DSA:3.30.70.380. Fdx_AntiC_bd. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01890.
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. PheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_RICPR

Accession

Primary (citable) accession number: Q9ZDB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Rickettsia prowazekii

Rickettsia prowazekii (strain Madrid E): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents