Q9ZD92 (LON_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	RP450

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	Lon protease		PRO_0000076145
Regions
	Domain	6 – 203	198	Lon
	Nucleotide binding	356 – 363	8	ATP By similarity
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZD92 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F38D1891B8ECA1F9	FASTA	784	88,071
	10 20 30 40 50 60 MNKKSLPLMA LRDMVVFPGV IAPIFVGRKK SLQALSRTTI SEENNSKYIL VTLQKKFDQE 70 80 90 100 110 120 NPSKHELYNT AILAKIIQIV KLPNNTAKIL IEAVARVKLS NIKDEEAFEA NYEIIPDEEI 130 140 150 160 170 180 LDIHNMRSLV DNAVQLFSKY AMNDKKVNAE IIETINKEIS NRTNFINIIN ILSSHLITSL 190 200 210 220 230 240 ETKQQLLEET NPVKRITTVI TTLTSNIVNS ETEHALQQRV RKQIEKTQRD YYLHEQMKAI 250 260 270 280 290 300 QKELDEDKSE LADIEKKIKS LKLSKEAKEK AESEFKKLRA MNQMSAESGV TRNYLETLLS 310 320 330 340 350 360 LPWGKYDNSK IDINQAEKIL NRDHFGLEKV KERIIEYLAV LQRSSKIRGP ILCLIGPPGV 370 380 390 400 410 420 GKTSLVKSIA EGMGRKYTKF SLGGVRDEAE IRGHRKTYLG SMPGKILGQL KKIKTSNPVM 430 440 450 460 470 480 LLDEIDKMSS DFRGDPASAL LEVLDPEQNS HFVDHYLEVE YDLSNVVFIA TANSHDLPRA 490 500 510 520 530 540 LSDRMEKIYI SGYVEEEKLQ IAKNYLVPKQ FKMHKIKEDE ITISEAAILD LIRYYTKESG 550 560 570 580 590 600 VRALEREICA LTRKALKQIL ANKTVKHISI DSNNLEEFLG AKKYNFGLAE KEDQIGSTTG 610 620 630 640 650 660 LAYTEVGGEL LTIEALAFSG KGEIKTTGKL GDVMKESAMA AYSCFRSRAT NFGLKYDNYK 670 680 690 700 710 720 DFDIHIHVPA GAIPKDGPSA GCALFTTIVS LMTKIPVHRT VAMTGEITLR GNVLPIGGLK 730 740 750 760 770 780 EKLLAASRGG IKTVLIPEEN VKDLKDIPPN IKENLEIISV SNIDQVLKHA LVEMPINKGL SYDL

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14907.1.
PIR	A71704.
RefSeq	NP_220831.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZD92.
SMR	Q9ZD92. Positions 594-775.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883360.
GenomeReviews	Gene locus RP450 in contig AJ235269_GR.
KEGG	rpr:RP450.
PATRIC	17901681. VBIRicPro72556_0463.
Phylogenomic databases
HOGENOM	HBG566281.
OMA	FMEGEIS.
ProtClustDB	CLSK870932.
Enzyme and pathway databases
BioCyc	RPRO272947:RP450-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_RICPR

Accession

Primary (citable) accession number: Q9ZD92

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program