Q9ZD77 (HEM3_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	RP466

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260
Subunit structure	Monomer By similarity.
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethane group By similarity. HAMAP-Rule MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Porphobilinogen deaminase HAMAP-Rule MF_00260		PRO_0000142982
Amino acid modifications
	Modified residue	242	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZD77 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 888878839DBFE155	FASTA	299	33,596
	10 20 30 40 50 60 MINSIRIGTR NSTLALIQTN LVIEQIKQIF PDINCEIVPI ITSGDLIQNK PLYDIGGKAL 70 80 90 100 110 120 FLKEIEQALL DKKIDLAVHS LKDIPGKIPV ELVIAAVLER EDPRDVLVCL NYQSIETLPQ 130 140 150 160 170 180 NAVIGSSAVR RKAFIKKIRP DLNIKVFRGN VDSRIKKLMT GEVDAIILSY AGLKRLNVFN 190 200 210 220 230 240 QKYCHLIEYS KMLPCIGQGV IAVEIRKDDN AMFNICSQIN HLPTFELIKP ERAFLEYLDA 250 260 270 280 290 NCSTPIAAYA QYLDAYNIQI DFMLGNLDCT KIIFQTEITN IKTSKICGIK AAKMMLAQQ

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235271 Genomic DNA. Translation: CAA14922.1.
PIR	H71705.
RefSeq	NP_220846.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZD77.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP466.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14922; CAA14922; CAA14922.
GeneID	883373.
KEGG	rpr:RP466.
PATRIC	17901713. VBIRicPro72556_0478.
Phylogenomic databases
eggNOG	COG0181.
HOGENOM	HOG000228587.
KO	K01749.
OMA	CLAERAF.
ProtClustDB	PRK00072.
Enzyme and pathway databases
UniPathway	UPA00251; UER00319.
Family and domain databases
Gene3D	3.30.160.40. 1 hit.
HAMAP	MF_00260. Porphobil_deam.
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
PANTHER	PTHR11557. PTHR11557. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. hemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_RICPR

Accession

Primary (citable) accession number: Q9ZD77

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program