Q9ZD46 (TRUB_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative tRNA pseudouridine synthase B
EC=5.4.99.25

Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase

Gene names

Name:	truB
Ordered Locus Names:	RP501

Organism

Rickettsia prowazekii (strain Madrid E) [Reference proteome] [HAMAP]

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia prowazekii

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080
Catalytic activity	tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080
Sequence similarities	Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.
Caution	Could be the product of a pseudogene. C-terminally truncated compared to other bacterial TruB.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	Putative tRNA pseudouridine synthase B HAMAP-Rule MF_01080		PRO_0000121895
Sites
	Active site	39	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ZD46 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 66B2F753BDA8566C	FASTA	172	19,116
	10 20 30 40 50 60 MGNYWLNFYK PRGISSAKLV NIVKKIIGKT KIGHAGTLDV EAEGILPLAV GEATKLIQLL 70 80 90 100 110 120 IDARKTYIFS VKFGTQTDSG DYTGKVIASK DYIPSQEEVY TVCSKFIGNI RQVPPIFSAI 130 140 150 160 170 KVNGVRAYKL AREGKIVELK PRNITIYDLK CLNFDKENAI ATYYTECSKG LI

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235272 Genomic DNA. Translation: CAA14953.1.
PIR	G71653.
RefSeq	NP_220877.1. NC_000963.1.
3D structure databases
ProteinModelPortal	Q9ZD46.
ModBase	Search...
Protein-protein interaction databases
STRING	272947.RP501.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAA14953; CAA14953; CAA14953.
GeneID	883154.
KEGG	rpr:RP501.
PATRIC	17901784. VBIRicPro72556_0510.
Phylogenomic databases
eggNOG	COG0130.
KO	K03177.
OMA	NAIATYY.
Family and domain databases
HAMAP	MF_01080. TruB_bact. Divergent sequence.
InterPro	IPR002501. PsdUridine_synth. IPR020103. PsdUridine_synth_cat_dom. IPR014780. tRNA_psdUridine_synth_TruB. [Graphical view]
PANTHER	PTHR13767. PTHR13767. 1 hit.
Pfam	PF01509. TruB_N. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00431. TruB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUB_RICPR

Accession

Primary (citable) accession number: Q9ZD46

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program