Reviewed, UniProtKB/Swiss-Prot Q9ZCZ1 (FAD3_RICPR)

Names and origin

Protein names

Recommended name:
    Putative fatty acid oxidation complex trifunctional enzyme
Including the following 2 domains:
    1- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35
    2- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase
              EC=4.2.1.17
              EC=5.3.3.8

Gene names

Ordered Locus Names:

RP560

Organism

Rickettsia prowazekii [Complete proteome] [HAMAP]

Taxonomic identifier

782 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
Sequence similarities	In the N-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: EC enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Putative fatty acid oxidation complex trifunctional enzyme		PRO_0000285698
Regions
	Region	1 – 384	384	3-hydroxyacyl-CoA dehydrogenase
	Region	453 – 720	268	Enoyl-CoA hydratase/isomerase

Sequences

	Sequence		Length	Mass (Da)
	Q9ZCZ1-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BAEC37A40044234E	FASTA	720	81,403
	10 20 30 40 50 60 MQNEIKKVCV IGAGVMGSGI AALIANSSHQ VVLLDILDKD SNDPNKIVKN SVKNLHKQKL 70 80 90 100 110 120 SPLSFPDKVN FITIGNLEHD LDLIKECNLV IEVIVEKLEI KHQLYNKIIP YLKEDAIIAS 130 140 150 160 170 180 NTSTLPLKKL KVNLPNNIKS RFVITHFFNP PRYMELVELI IDHTIKDEVI EKVSVFLTKM 190 200 210 220 230 240 LGKTIIKCND TPGFIANRVG CFLLELVVHK AIAQNLNFVT IDQIFSRCLG LPNTGIFGLY 250 260 270 280 290 300 DLIGHDVMKL ISSSLISALP KSDDYHRIYT NTKVFDKMIE HNLIGRKGEG GFYRLSVSNG 310 320 330 340 350 360 KKIKEVINIS DLSYHPVQKV DISFNSLNEL LSSNSIYGKF FSEIITEFYI YLTSLVPSVT 370 380 390 400 410 420 NNIYDIDTTM KLGYSWHYGP FELLTIAVKN GWNLIIKNAD LMNIPLPKYL ASKEYQKIDK 430 440 450 460 470 480 QKFNSKKDFL QESKIVLSND SANLIHYCEN LVFVITTKMN SLNHNVFYLL QEAVSKAENY 490 500 510 520 530 540 GKNLYIYPQG NNFSAGADLK LILSYIQDGN FHNLENLLKL GQQTMRYLKY SSVHIISCAR 550 560 570 580 590 600 GVALGGGCEL LLNSSYIVAN QELNAGLIEL GVGLIPGWTG VTEMFARSNG NKTKLIRNIK 610 620 630 640 650 660 NIIEQNKTSS ADYFKADYGI KNMQVNMNKH YILDDALKLR ISKKIVSIPN KITLPKINIV 670 680 690 700 710 720 SEIDTSKYNE LQNKVLNKFQ NIIDKHNEIS EAELLTYERE MFLELAKTPQ TIEKLQAIVG

References

[1]

"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ235272 Genomic DNA. Translation: CAA15008.1.
PIR	F71660.
RefSeq	NP_220931.1.
3D structure databases
HSSP	HSSP built from PDB template 3HDH based on UniProtKB P00348.
ModBase	Search...
Genome annotation databases
GeneID	883251.
GenomeReviews	Gene locus RP560 in contig AJ235269_GR.
KEGG	rpr:RP560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9ZCZ1.
OMA	Q9ZCZ1. FRTLDVV.
Enzyme and pathway databases
BioCyc	RPRO272947:RP560-MON.
BRENDA	1.1.1.35. 141977. 4.2.1.17. 141977. 5.3.3.8. 141977.
Family and domain databases
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00067. 3HCDH. False negative. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAD3_RICPR

Accession

Primary (citable) accession number: Q9ZCZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)