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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51SubstrateUniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi59Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi62Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi64MagnesiumUniRule annotation1
Binding sitei100SubstrateUniRule annotation1
Binding sitei102S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Queuosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:RP607
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004162131 – 2347-carboxy-7-deazaguanine synthaseAdd BLAST234

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272947.RP607.

Structurei

3D structure databases

ProteinModelPortaliQ9ZCV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 38Substrate bindingUniRule annotation3
Regioni144 – 146S-adenosyl-L-methionine bindingUniRule annotation3
Regioni195 – 198S-adenosyl-L-methionine bindingUniRule annotation4

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EIZ. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000266142.
OMAiHRFLQPM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE. 1 hit.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ZCV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQRINSYKM FGHNPKRSIL SGDGTKLEVQ SIFKTIQGEG IFVGYPSIFI
60 70 80 90 100
RLGGCNLACN FCDTEFEDFK LIDIDQILNK VKNLSLNSKN AKTINLVVIT
110 120 130 140 150
GGEPMRQPIG LLCQKLLDQD FKVQIETNGT LYRSLPKEVF IVCSPKVGKT
160 170 180 190 200
GYNKIREDLL PQISAVKFII SKNIVEYSII PEVGQSAYDI PVFVQSMDQN
210 220 230
DKRLNNENNE LAVKLALESG ARLSLQTHKF LGIE
Length:234
Mass (Da):26,247
Last modified:May 1, 1999 - v1
Checksum:i1BC9DAEE2E04C64B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235272 Genomic DNA. Translation: CAA15051.1.
PIRiA71666.
RefSeqiNP_220975.1. NC_000963.1.
WP_010886333.1. NC_000963.1.

Genome annotation databases

EnsemblBacteriaiCAA15051; CAA15051; CAA15051.
GeneIDi883801.
KEGGirpr:RP607.
PATRICi17902031. VBIRicPro72556_0626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235272 Genomic DNA. Translation: CAA15051.1.
PIRiA71666.
RefSeqiNP_220975.1. NC_000963.1.
WP_010886333.1. NC_000963.1.

3D structure databases

ProteinModelPortaliQ9ZCV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15051; CAA15051; CAA15051.
GeneIDi883801.
KEGGirpr:RP607.
PATRICi17902031. VBIRicPro72556_0626.

Phylogenomic databases

eggNOGiENOG4105EIZ. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000266142.
OMAiHRFLQPM.

Enzyme and pathway databases

UniPathwayiUPA00391.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE. 1 hit.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEE_RICPR
AccessioniPrimary (citable) accession number: Q9ZCV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.